The Journal of Experimental Medicine
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Journal of Experimental Medicine, Vol 169, 315-320, Copyright © 1989 by Rockefeller University Press

ARTICLES

Clonal expansion of human T lymphocytes initiated by dendritic cells

E Langhoff and RM Steinman
Laboratory of Cellular Physiology and Immunology, Rockefeller University, New York, New York.

The accessory cell requirements for cloning T cells in the presence of lectin and T cell growth factors were examined with cells from human peripheral blood. We found that dendritic cells were active and perhaps essential. Single CD4+ lymphocytes could be cloned with 80% efficiency, and CD8+ cells with 50-60% efficiency if 10(3) syngeneic or allogeneic dendritic cells were added. Some T cell clones developed even with one dendritic cell. Monocytes or B lymphocytes from blood were at least 100- fold weaker in supporting clonal growth. These findings suggest a specialized feeder cell requirement, namely dendritic cells, for cloning T lymphocytes from single resting precursors.
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