Distinct patterns of heavy chain variable region subgroup use by human monoclonal autoantibodies of different specificity

doi:10.1084/jem.168.6.2361

This Article

	Full Text (PDF)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Silverman, G. J.
	Articles by Carson, D. A.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Silverman, G. J.
	Articles by Carson, D. A.


Social Bookmarking

	What's this?

ARTICLES

Distinct patterns of heavy chain variable region subgroup use by human monoclonal autoantibodies of different specificity

GJ Silverman, F Goni, J Fernandez, PP Chen, B Frangione and DA Carson
Department of Basic and Clinical Research, Research Institute of Scripps Clinic, La Jolla, California 92037.

Using a panel of antibodies specific for H and L chain variable region subgroups, a panel of human monoclonal cold agglutinin (CA) and rheumatoid factor (RF) autoantibodies were analyzed. The vast majority of the two types of autoantibodies utilized VkIII L chains, many of which probably derive from the Humkv325 gene. However, while most RFs (77%) utilized VHI H chains, all the CAs used VHII subgroup H chains. These results are consistent with a model of autoantibody generation, wherein binding specificity is H chain defined in a set of antibodies that use a multipotential L chain.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Widhopf, G. F. II, Goldberg, C. J., Toy, T. L., Rassenti, L. Z., Wierda, W. G., Byrd, J. C., Keating, M. J., Gribben, J. G., Rai, K. R., Kipps, T. J. (2008). Nonstochastic pairing of immunoglobulin heavy and light chains expressed by chronic lymphocytic leukemia B cells is predicated on the heavy chain CDR3. Blood 111: 3137-3144 [Abstract] [Full Text]
Widhopf, G. F. II, Rassenti, L. Z., Toy, T. L., Gribben, J. G., Wierda, W. G., Kipps, T. J. (2004). Chronic lymphocytic leukemia B cells of more than 1% of patients express virtually identical immunoglobulins. Blood 104: 2499-2504 [Abstract] [Full Text]
Widhopf, G. F. II, Brinson, D. C., Kipps, T. J., Tighe, H. (2004). Transgenic Expression of a Human Polyreactive Ig Expressed in Chronic Lymphocytic Leukemia Generates Memory-Type B Cells That Respond to Nonspecific Immune Activation. J. Immunol. 172: 2092-2099 [Abstract] [Full Text]
Widhopf, G. F. II, Kipps, T. J. (2001). Normal B Cells Express 51p1-Encoded Ig Heavy Chains That Are Distinct From Those Expressed by Chronic Lymphocytic Leukemia B Cells. J. Immunol. 166: 95-102 [Abstract] [Full Text]
Cauerhff, A., Braden, B. C., Carvalho, J. G., Aparicio, R., Polikarpov, I., Leoni, J., Goldbaum, F. A. (2000). Three-Dimensional Structure of the Fab from a Human IgM Cold Agglutinin. J. Immunol. 165: 6422-6428 [Abstract] [Full Text]
Chang, T. Y., Siegel, D. L. (1998). Genetic and Immunological Properties of Phage-Displayed Human Anti-Rh(D) Antibodies: Implications for Rh(D) Epitope Topology. Blood 91: 3066-3078 [Abstract] [Full Text]
Welschof, M., Terness, P., Kipriyanov, S. M., Stanescu, D., Breitling, F., Dorsam, H., Dubel, S., Little, M., Opelz, G. (1997). The antigen-binding domain of a human IgG-anti-F(ab')2�autoantibody. Proc. Natl. Acad. Sci. USA 94: 1902-1907 [Abstract] [Full Text]
Kieber-Emmons, T., Von Feldt, J. M., Godillot, A. P., McCallus, D., Srikantan, V., Weiner, D. B., Williams, W. V. (1994). Isolated VH4 Heavy Chain Variable Regions Bind DNA Characterization of a Recombinant Antibody Heavy Chain Library Derived from Patient(s) with Active SLE. Lupus 3: 379-392 [Abstract]