Feedback inhibition of immunoglobulin gene rearrangement by membrane mu, but not by secreted mu heavy chains [published erratum appears in J Exp Med 1989 Jun 1;169(6):2269]

doi:10.1084/jem.168.4.1363

This Article

	Full Text (PDF)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Manz, J.
	Articles by Storb, U.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Manz, J.
	Articles by Storb, U.


Social Bookmarking

	What's this?

ARTICLES

Feedback inhibition of immunoglobulin gene rearrangement by membrane mu, but not by secreted mu heavy chains [published erratum appears in J Exp Med 1989 Jun 1;169(6):2269]

J Manz, K Denis, O Witte, R Brinster and U Storb
Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.

Previous work (6-10) has shown that allelic exclusion of Ig gene expression is controlled by functionally rearranged mu and kappa genes. This report deals with the comparison of membrane mu (micron) and secreted mu (microsecond) in promoting such feedback inhibition. Splenic B cell hybridomas were analyzed from transgenic mice harboring a rearranged kappa gene alone or in combination with either an intact rearranged mu gene or a truncated version of the mu gene. The intact mu gene is capable of producing both membrane and secreted forms of the protein, while the truncated version can only encode the secreted form. The role of the microsecond was also tested in pre-B cell lines. Analysis of the extent of endogenous Ig gene rearrangement revealed that (a) the production of micron together with kappa can terminate Ig gene rearrangement; (b) microsecond with kappa does not have this feedback effect; (c) microsecond may interfere with the effect of micron and kappa; and (d) the feedback shown here probably represents a complete shutoff of the specific recombinase by micron + kappa; the data do not address the question of mu alone affecting the accessibility of H genes for rearrangement.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Sirac, C., Carrion, C., Duchez, S., Comte, I., Cogne, M. (2006). Light chain inclusion permits terminal B cell differentiation and does not necessarily result in autoreactivity. Proc. Natl. Acad. Sci. USA 103: 7747-7752 [Abstract] [Full Text]
Fuentes-Panana, E. M., Bannish, G., Shah, N., Monroe, J. G. (2004). Basal Ig{alpha}/Ig{beta} Signals Trigger the Coordinated Initiation of Pre-B Cell Antigen Receptor-Dependent Processes. J. Immunol. 173: 1000-1011 [Abstract] [Full Text]
Yuan, D., Bibi, R., Dang, T. (2004). The role of adjuvant on the regulatory effects of NK cells on B cell responses as revealed by a new model of NK cell deficiency. Int Immunol 16: 707-716 [Abstract] [Full Text]
Quong, M. W., Martensson, A., Langerak, A. W., Rivera, R. R., Nemazee, D., Murre, C. (2004). Receptor Editing and Marginal Zone B Cell Development Are Regulated by the Helix-Loop-Helix Protein, E2A. J. Exp. Med. 199: 1101-1112 [Abstract] [Full Text]
Ravirajan, C T, Isenberg, D A (2002). Transgenic models of tolerance and autoimmunity: with special reference to systemic lupus erythematosus. Lupus 11: 843-849 [Abstract]
Shimizu, T., Mundt, C., Licence, S., Melchers, F., Martensson, I.-L. (2002). VpreB1/VpreB2/{lambda}5 Triple-Deficient Mice Show Impaired B Cell Development but Functional Allelic Exclusion of the IgH Locus. J. Immunol. 168: 6286-6293 [Abstract] [Full Text]
Barreto, V., Meo, T., Cumano, A. (2001). Mice Triallelic for the Ig Heavy Chain Locus: Implications for VHDJH Recombination. J. Immunol. 166: 5638-5645 [Abstract] [Full Text]
Mundt, C., Licence, S., Shimizu, T., Melchers, F., Martensson, I.-L. (2001). Loss of Precursor B Cell Expansion but Not Allelic Exclusion in VpreB1/VpreB2 Double-deficient Mice. J. Exp. Med. 193: 435-446 [Abstract] [Full Text]
Maki, K., Nagata, K., Kitamura, F., Takemori, T., Karasuyama, H. (2000). Immunoglobulin {beta} Signaling Regulates Locus Accessibility for Ordered Immunoglobulin Gene Rearrangements. J. Exp. Med. 191: 1333-1340 [Abstract] [Full Text]
Kenny, J. J., Rezanka, L. J., Lustig, A., Fischer, R. T., Yoder, J., Marshall, S., Longo, D. L. (2000). Autoreactive B Cells Escape Clonal Deletion by Expressing Multiple Antigen Receptors. J. Immunol. 164: 4111-4119 [Abstract] [Full Text]
Shen, X., Bozek, G., Pinkert, C. A., Storb, U. (1999). The CH1 and transmembrane domains of {micro} in the context of a {gamma}2b transgene do not suffice to promote B cell maturation. Int Immunol 11: 1663-1671 [Abstract] [Full Text]
Watanabe, N., Nisitani, S., Ikuta, K., Suzuki, M., Chiba, T., Honjo, T. (1999). Expression Levels of B Cell Surface Immunoglobulin Regulate Efficiency of Allelic Exclusion and Size of Autoreactive B-1 Cell Compartment. J. Exp. Med. 190: 461-470 [Abstract] [Full Text]
Benlagha, K., Guglielmi, P., Cooper, M. D., Lassoued, K. (1999). Modifications of Igalpha and Igbeta Expression as a Function of B Lineage Differentiation. J. Biol. Chem. 274: 19389-19396 [Abstract] [Full Text]
Tachibana, H., Haruta, H., Yamada, K. (1999). Light Chain Shifting: Identification of a Human Plasma Cell Line Actively Undergoing Light Chain Replacement. Blood 93: 198-207 [Abstract] [Full Text]
Retter, M. W., Nemazee, D. (1998). Receptor Editing Occurs Frequently during Normal B Cell Development. J. Exp. Med. 188: 1231-1238 [Abstract] [Full Text]
Tornberg, U.-C., Bergqvist, I., Haury, M., Holmberg, D. (1998). Regulation of B Lymphocyte Development by the Truncated Immunoglobulin Heavy Chain Protein D�. J. Exp. Med. 187: 703-709 [Abstract] [Full Text]
Malone, C. S., Omori, S. A., Wall, R. (1997). Silencer elements controlling the B29 (Igbeta ) promoter are neither promoter- nor�cell-type-specific. Proc. Natl. Acad. Sci. USA 94: 12314-12319 [Abstract] [Full Text]
Gong, S., Sanchez, M., Nussenzweig, M. C. (1996). Counterselection against D� Is Mediated through Immunoglobulin (Ig)alpha -Igbeta. J. Exp. Med. 184: 2079-2084 [Abstract] [Full Text]
Tachibana, H., Ushio, Y., Krungkasem, C., Shirahata, S. (1996). Inducing the Loss of Immunoglobulin &lgr;Light Chain Production and the Rearrangement of a Previously Excluded Allele in Human Plasma B Cell Lines with Concanavalin A. J. Biol. Chem. 271: 17404-17410 [Abstract] [Full Text]
Papavasiliou, F, Misulovin, Z, Suh, H, Nussenzweig, M. (1995). The role of Ig beta in precursor B cell transition and allelic exclusion. Science 268: 408-411 [Abstract]
Spanopoulou, E, Roman, C A, Corcoran, L M, Schlissel, M S, Silver, D P, Nemazee, D, Nussenzweig, M C, Shinton, S A, Hardy, R R, Baltimore, D (1994). Functional immunoglobulin transgenes guide ordered B-cell differentiation in Rag-1-deficient mice.. Genes Dev. 8: 1030-1042 [Abstract]
Goodnow, C., Adelstein, S, Basten, A (1990). The need for central and peripheral tolerance in the B cell repertoire. Science 248: 1373-1379 [Abstract]
Pursel, V., Pinkert, C., Miller, K., Bolt, D., Campbell, R., Palmiter, R., Brinster, R., Hammer, R. (1989). Genetic engineering of livestock. Science 244: 1281-1288 [Abstract]