The Journal of Experimental Medicine
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Journal of Experimental Medicine, Vol 168, 933-940, Copyright © 1988 by Rockefeller University Press

ARTICLES

Extensive deletions and insertions in different MHC supratypes detected by pusled field gel electrophoresis

K Tokunaga, G Saueracker, PH Kay, FT Christiansen, R Anand and RL Dawkins
Department of Clinical Immunology, Royal Perth Hospital, Perth, Western Australia.

The genomic organization of the human MHC was examined in multiple examples of six different supratypes using pulsed field electrophoresis (PFGE) after digestion of genomic DNA with infrequency cutting restriction endonucleases. Differences in restriction fragment length and band intensity were shown to be specific for each supratype. Mapping of the MHC revealed that each supratype contains previously undescribed deletions and insertions between HLA B and DQ regions.
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