Purification and characterization of eight class 5 outer membrane protein variants from a clone of Neisseria meningitidis serogroup A

doi:10.1084/jem.168.2.507

This Article

	Full Text (PDF)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Achtman, M.
	Articles by Moll, A.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Achtman, M.
	Articles by Moll, A.


Social Bookmarking

	What's this?

ARTICLES

Purification and characterization of eight class 5 outer membrane protein variants from a clone of Neisseria meningitidis serogroup A

M Achtman, M Neibert, BA Crowe, W Strittmatter, B Kusecek, E Weyse, MJ Walsh, B Slawig, G Morelli and A Moll
Max-Planck Institut fur Molekulare Genetik, Berlin, Federal Republic of Germany.

Methods published for the purification of P.II proteins from Neisseria gonorrhoea have been modified to allow the purification of class 5 proteins from Neisseria meningitidis serogroup A bacteria. The five class 5 protein electrophoretic variants detected within an epidemic in the Gambia (a, b, c, d, and e) and three other variants (f, g, and h) found within other isolates of the same clone in West Africa have been purified with yields of 6-28 mg. The NH2-terminal amino acid sequence for variant c differs from those of the other class 5 proteins, whereas the latter are very similar to the sequence predicted for two class 5 proteins from DNA analyses of serogroup C meningococci and determined for 8 P.II proteins from gonococci. Numerous other regulatory, chemical, and serological differences were found between the c protein and the other class 5 proteins such that we recommend that the class 5 proteins be subdivided into two subclasses. mAbs have been isolated that distinguish between these two protein subclasses and Western blotting with these antibodies enabled us to conclude that both protein subclasses were found in bacteria isolated from different epidemics and pandemics of the last 50 yr.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Higashi, D. L., Lee, S. W., Snyder, A., Weyand, N. J., Bakke, A., So, M. (2007). Dynamics of Neisseria gonorrhoeae Attachment: Microcolony Development, Cortical Plaque Formation, and Cytoprotection. Infect. Immun. 75: 4743-4753 [Abstract] [Full Text]
Lee, H. S. W., Boulton, I. C., Reddin, K., Wong, H., Halliwell, D., Mandelboim, O., Gorringe, A. R., Gray-Owen, S. D. (2007). Neisserial Outer Membrane Vesicles Bind the Coinhibitory Receptor Carcinoembryonic Antigen-Related Cellular Adhesion Molecule 1 and Suppress CD4+ T Lymphocyte Function. Infect. Immun. 75: 4449-4455 [Abstract] [Full Text]
Simms, A. N., Jerse, A. E. (2006). In Vivo Selection for Neisseria gonorrhoeae Opacity Protein Expression in the Absence of Human Carcinoembryonic Antigen Cell Adhesion Molecules.. Infect. Immun. 74: 2965-2974 [Abstract] [Full Text]
Plant, L., Sundqvist, J., Zughaier, S., Lovkvist, L., Stephens, D. S., Jonsson, A.-B. (2006). Lipooligosaccharide Structure Contributes to Multiple Steps in the Virulence of Neisseria meningitidis. Infect. Immun. 74: 1360-1367 [Abstract] [Full Text]
Plant, L. J., Jonsson, A.-B. (2006). Type IV Pili of Neisseria gonorrhoeae Influence the Activation of Human CD4+ T Cells. Infect. Immun. 74: 442-448 [Abstract] [Full Text]
Moore, J., Bailey, S. E. S., Benmechernene, Z., Tzitzilonis, C., Griffiths, N. J. E., Virji, M., Derrick, J. P. (2005). Recognition of Saccharides by the OpcA, OpaD, and OpaB Outer Membrane Proteins from Neisseria meningitidis. J. Biol. Chem. 280: 31489-31497 [Abstract] [Full Text]
Estabrook, M. M., Jack, D. L., Klein, N. J., Jarvis, G. A. (2004). Mannose-Binding Lectin Binds to Two Major Outer Membrane Proteins, Opacity Protein and Porin, of Neisseria meningitidis. J. Immunol. 172: 3784-3792 [Abstract] [Full Text]
Dunn, K. L. R., Farrant, J. L., Langford, P. R., Kroll, J. S. (2003). Bacterial [Cu,Zn]-Cofactored Superoxide Dismutase Protects Opsonized, Encapsulated Neisseria meningitidis from Phagocytosis by Human Monocytes/Macrophages. Infect. Immun. 71: 1604-1607 [Abstract] [Full Text]
Prince, S. M., Achtman, M., Derrick, J. P. (2002). Crystal structure of the OpcA integral membrane adhesin from Neisseriameningitidis. Proc. Natl. Acad. Sci. USA 99: 3417-3421 [Abstract] [Full Text]
Muenzner, P., Billker, O., Meyer, T. F., Naumann, M. (2002). Nuclear Factor-kappa B Directs Carcinoembryonic Antigen-related Cellular Adhesion Molecule 1 Receptor Expression in Neisseria gonorrhoeae-infected Epithelial Cells. J. Biol. Chem. 277: 7438-7446 [Abstract] [Full Text]
Takahashi, H., Watanabe, H. (2002). A broad-host-range vector of incompatibility group Q can work as a plasmid vector in Neisseria meningitidis: a new genetical tool. Microbiology 148: 229-236 [Abstract] [Full Text]
Pelicic, V., Morelle, S., Lampe, D., Nassif, X. (2000). Mutagenesis of Neisseria meningitidis by In Vitro Transposition of Himar1 mariner. J. Bacteriol. 182: 5391-5398 [Abstract] [Full Text]
Muenzner, P., Dehio, C., Fujiwara, T., Achtman, M., Meyer, T. F., Gray-Owen, S. D. (2000). Carcinoembryonic Antigen Family Receptor Specificity of Neisseria meningitidis Opa Variants Influences Adherence to and Invasion of Proinflammatory Cytokine-Activated Endothelial Cells. Infect. Immun. 68: 3601-3607 [Abstract] [Full Text]
Song, W., Ma, L., Chen, R., Stein, D. C. (2000). Role of Lipooligosaccharide in Opa-independent Invasion of Neisseria gonorrhoeae into Human Epithelial Cells. J. Exp. Med. 191: 949-960 [Abstract] [Full Text]
Jerse, A. E. (1999). Experimental Gonococcal Genital Tract Infection and Opacity Protein Expression in Estradiol-Treated Mice. Infect. Immun. 67: 5699-5708 [Abstract] [Full Text]
Marceau, M., Nassif, X. (1999). Role of Glycosylation at Ser63 in Production of Soluble Pilin in Pathogenic Neisseria. J. Bacteriol. 181: 656-661 [Abstract] [Full Text]
Eberhard, T., Virkola, R., Korhonen, T., Kronvall, G., Ullberg, M. (1998). Binding to Human Extracellular Matrix by Neisseria meningitidis. Infect. Immun. 66: 1791-1794 [Abstract] [Full Text]
Estabrook, M. M., Zhou, D., Apicella, M. A. (1998). Nonopsonic Phagocytosis of Group C Neisseria meningitidis by Human Neutrophils. Infect. Immun. 66: 1028-1036 [Abstract] [Full Text]
Malorny, B., Morelli, G., Kusecek, B., Kolberg, J., Achtman, M. (1998). Sequence Diversity, Predicted Two-Dimensional Protein Structure, and Epitope Mapping of Neisserial Opa Proteins. J. Bacteriol. 180: 1323-1330 [Abstract] [Full Text]
Muenzner, P., Naumann, M., Meyer, T. F., Gray-Owen, S. D. (2001). Pathogenic Neisseria Trigger Expression of Their Carcinoembryonic Antigen-related Cellular Adhesion Molecule 1 (CEACAM1; Previously CD66a) Receptor on Primary Endothelial Cells by Activating the Immediate Early Response Transcription Factor, Nuclear Factor-kappa B. J. Biol. Chem. 276: 24331-24340 [Abstract] [Full Text]