Two rare populations of mouse Thy-1lo bone marrow cells repopulate the thymus

doi:10.1084/jem.167.5.1671

This Article

	Full Text (PDF)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Spangrude, G. J.
	Articles by Weissman, I. L.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Spangrude, G. J.
	Articles by Weissman, I. L.


Social Bookmarking

	What's this?

ARTICLES

Two rare populations of mouse Thy-1lo bone marrow cells repopulate the thymus

GJ Spangrude, CE Muller-Sieburg, S Heimfeld and IL Weissman
Department of Pathology, Stanford University School of Medicine, California 94305.

Two-color FACS analysis of mouse bone marrow reveals a rare population, comprising 0.1-0.3% of the total, that expresses low levels of the Thy- 1 antigen but does not express any of five surface markers that characterize differentiated hematolymphoid cells. We demonstrate here that this fraction of mouse bone marrow is enormously enriched in cells that can home to the thymus and differentiate into mature T lymphocytes, subsequently migrating to peripheral lymphoid organs. Only a subset of the FACS-isolated fraction (1/90 after intrathymic injection) is capable of responding to the thymic microenvironment with a productive commitment to the T cell lineage. A second fraction of mouse bone marrow, which expresses how levels of Thy-1 but is also positive for at least one of five hematolymphoid lineage-specific markers, also contains cells that home to the thymus and establish colonies of thymocytes. The two fractions each contribute approximately equal amounts of thymic colony-forming units (CFUt) to the bone marrow, and together can account for at least half of the CFUt in whole bone marrow.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Cho, R. H., Sieburg, H. B., Muller-Sieburg, C. E. (2008). A new mechanism for the aging of hematopoietic stem cells: aging changes the clonal composition of the stem cell compartment but not individual stem cells. Blood 111: 5553-5561 [Abstract] [Full Text]
Perry, S. S., Zhao, Y., Nie, L., Cochrane, S. W., Huang, Z., Sun, X.-H. (2007). Id1, but not Id3, directs long-term repopulating hematopoietic stem-cell maintenance. Blood 110: 2351-2360 [Abstract] [Full Text]
Petrie, H. T., Kincade, P. W. (2005). Many roads, one destination for T cell progenitors. J. Exp. Med. 202: 11-13 [Abstract] [Full Text]
Muller-Sieburg, C. E., Cho, R. H., Karlsson, L., Huang, J.-F., Sieburg, H. B. (2004). Myeloid-biased hematopoietic stem cells have extensive self-renewal capacity but generate diminished lymphoid progeny with impaired IL-7 responsiveness. Blood 103: 4111-4118 [Abstract] [Full Text]
Fortin, M., Steff, A.-M., Felberg, J., Ding, I., Schraven, B., Johnson, P., Hugo, P. (2002). Apoptosis Mediated Through CD45 Is Independent of Its Phosphatase Activity and Association with Leukocyte Phosphatase-Associated Phosphoprotein. J. Immunol. 168: 6084-6089 [Abstract] [Full Text]
Lind, E. F., Prockop, S. E., Porritt, H. E., Petrie, H. T. (2001). Mapping Precursor Movement through the Postnatal Thymus Reveals Specific Microenvironments Supporting Defined Stages of Early Lymphoid Development. J. Exp. Med. 194: 127-134 [Abstract] [Full Text]
Trop, S., Charron, J., Arguin, C., Hugo, P. (2000). Thymic Selection Generates T Cells Expressing Self-Reactive TCRs in the Absence of CD45. J. Immunol. 165: 3073-3079 [Abstract] [Full Text]
Huss, R. (2000). Isolation of Primary and Immortalized CD34- Hematopoietic and Mesenchymal Stem Cells from Various Sources. Stem Cells 18: 1-9 [Abstract] [Full Text]
Dunon, D., Allioli, N., Vainio, O., Ody, C., Imhof, B.A. (1999). Quantification of T-Cell Progenitors During Ontogeny: Thymus Colonization Depends on Blood Delivery of Progenitors. Blood 93: 2234-2243 [Abstract] [Full Text]
Montecino-Rodriguez, E., Clark, R., Dorshkind, K. (1998). Effects of Insulin-Like Growth Factor Administration and Bone Marrow Transplantation on Thymopoiesis in Aged Mice. Endocrinology 139: 4120-4126 [Abstract] [Full Text]
Ito, M, Anan, K, Misawa, M, Kai, S, Hara, H (1996). In vitro differentiation of murine Sca-1+Lin- cells into myeloid, B cell and T cell lineages. Stem Cells 14: 412-418 [Abstract]
Prakapas, Z, Denoyelle, M, Dargemont, C, Kroese, F., Thiery, J., Deugnier, M. (1993). Enrichment and characterization of thymus-repopulating cells in stroma-dependent cultures of rat bone marrow. J. Cell Sci. 104: 1039-1048 [Abstract]
Gabbianelli, M, Sargiacomo, M, Pelosi, E, Testa, U, Isacchi, G, Peschle, C (1990). "Pure" human hematopoietic progenitors: permissive action of basic fibroblast growth factor. Science 249: 1561-1564 [Abstract]