Differential regulation of IgG1 and IgE synthesis by interleukin 4

doi:10.1084/jem.167.1.183

This Article

	Full Text (PDF)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Snapper, C. M.
	Articles by Paul, W. E.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Snapper, C. M.
	Articles by Paul, W. E.


Social Bookmarking

	What's this?

ARTICLES

Differential regulation of IgG1 and IgE synthesis by interleukin 4

CM Snapper, FD Finkelman and WE Paul
Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.

IL-4/B cell stimulatory factor-1 is a T cell-derived lymphokine that has been shown to enhance IgG1 and IgE and to suppress IgG3 and IgG2b secretion by B cells stimulated with bacterial LPS. We show here that the stimulation of IgG1 and IgE secretion in response to rIL-4 is differentially regulated. The dose-response curve for IgG1 production is bimodal with peaks at 100 and 10,000 U/ml. IgE production is modest at 100 U/ml and exhibits a progressive enhancement as the IL-4 concentration is increased to 10,000 U/ml, reaching approximately 1 microgram of IgE from an initial cell number of 2 X 10(4). Both of these effects are reversed by monoclonal anti-IL-4 antibody. Neither the enhancing nor suppressing effects of IL-4 can be explained by changes in viable cell yields or [3H]thymidine incorporation. The production of both IgG1 and IgE is controlled by IL-4 in a two-phase manner. During the initial 2 d of culture with LPS, IL-4 action for both IgG1 and IgE production is relatively concentration independent at doses greater than 600 U/ml. This 2-d treatment leads to maximal IgG1 production at day 6 with no further addition of IL-4. Addition of IL-4 during the final 4 d of culture has no effect at concentrations under 100 U/ml. At higher concentrations, IL-4 is strikingly suppressive for IgG1 production. By contrast, little IgE is produced unless IL-4 is present after 2 d of culture and the response is directly dependent on the concentration of IL-4 during this second phase of culture with maximal responses observed at 10,000 U/ml. These differences in IL-4 requirements for IgG1 and IgE production, respectively, may have an important role in the regulation of the synthesis of these isotypes in responses to microbial antigens.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Arlen, P. A., Singleton, M., Adamovicz, J. J., Ding, Y., Davoodi-Semiromi, A., Daniell, H. (2008). Effective Plague Vaccination via Oral Delivery of Plant Cells Expressing F1-V Antigens in Chloroplasts. Infect. Immun. 76: 3640-3650 [Abstract] [Full Text]
Doi, T., Obayashi, K., Kadowaki, T., Fujii, H., Koyasu, S. (2008). PI3K is a negative regulator of IgE production. Int Immunol 20: 499-508 [Abstract] [Full Text]
Obayashi, K., Doi, T., Koyasu, S. (2007). Dendritic cells suppress IgE production in B cells. Int Immunol 19: 217-226 [Abstract] [Full Text]
Ishizaki, K., Yamada, A., Yoh, K., Nakano, T., Shimohata, H., Maeda, A., Fujioka, Y., Morito, N., Kawachi, Y., Shibuya, K., Otsuka, F., Shibuya, A., Takahashi, S. (2007). Th1 and Type 1 Cytotoxic T Cells Dominate Responses in T-bet Overexpression Transgenic Mice That Develop Contact Dermatitis. J. Immunol. 178: 605-612 [Abstract] [Full Text]
Pongratz, G., McAlees, J. W., Conrad, D. H., Erbe, R. S., Haas, K. M., Sanders, V. M. (2006). The Level of IgE Produced by a B Cell Is Regulated by Norepinephrine in a p38 MAPK- and CD23-Dependent Manner.. J. Immunol. 177: 2926-2938 [Abstract] [Full Text]
Carey, J. B., Allshire, A., van Pelt, F. N. (2006). Immune Modulation by Cadmium and Lead in the Acute Reporter Antigen-Popliteal Lymph Node Assay. Toxicol Sci 91: 113-122 [Abstract] [Full Text]
Singh, U. P., Singh, S., Boyaka, P. N., McGhee, J. R., Lillard, J. W. Jr (2004). Granulocyte chemotactic protein-2 mediates adaptive immunity in part through IL-8R{beta} interactions. J. Leukoc. Biol. 76: 1240-1247 [Abstract] [Full Text]
Singh, U. P., Singh, S., Ravichandran, P., Taub, D. D., Lillard, J. W. Jr. (2004). Viral Macrophage-Inflammatory Protein-II: A Viral Chemokine That Differentially Affects Adaptive Mucosal Immunity Compared with Its Mammalian Counterparts. J. Immunol. 173: 5509-5516 [Abstract] [Full Text]
Mana, P., Goodyear, M., Bernard, C., Tomioka, R., Freire-Garabal, M., Linares, D. (2004). Tolerance induction by molecular mimicry: prevention and suppression of experimental autoimmune encephalomyelitis with the milk protein butyrophilin. Int Immunol 16: 489-499 [Abstract] [Full Text]
Nambu, Y., Sugai, M., Gonda, H., Lee, C.-G., Katakai, T., Agata, Y., Yokota, Y., Shimizu, A. (2003). Transcription-Coupled Events Associating with Immunoglobulin Switch Region Chromatin. Science 302: 2137-2140 [Abstract] [Full Text]
Cappello, P., Triebel, F., Iezzi, M., Caorsi, C., Quaglino, E., Lollini, P.-L., Amici, A., Di Carlo, E., Musiani, P., Giovarelli, M., Forni, G. (2003). LAG-3 Enables DNA Vaccination to Persistently Prevent Mammary Carcinogenesis in HER-2/neu Transgenic BALB/c Mice. Cancer Res. 63: 2518-2525 [Abstract] [Full Text]
Singh, R. R., Saxena, V., Zang, S., Li, L., Finkelman, F. D., Witte, D. P., Jacob, C. O. (2003). Differential Contribution of IL-4 and STAT6 vs STAT4 to the Development of Lupus Nephritis. J. Immunol. 170: 4818-4825 [Abstract] [Full Text]
Lillard, J. W. Jr, Singh, U. P., Boyaka, P. N., Singh, S., Taub, D. D., McGhee, J. R. (2003). MIP-1alpha and MIP-1beta differentially mediate mucosal and systemic adaptive immunity. Blood 101: 807-814 [Abstract] [Full Text]
Hanson, E. M., Dickensheets, H., Qu, C.-K., Donnelly, R. P., Keegan, A. D. (2003). Regulation of the Dephosphorylation of Stat6. PARTICIPATION OF TYR-713 IN THE INTERLEUKIN-4 RECEPTOR alpha , THE TYROSINE PHOSPHATASE SHP-1, AND THE PROTEASOME. J. Biol. Chem. 278: 3903-3911 [Abstract] [Full Text]
Grosfeld, H., Cohen, S., Bino, T., Flashner, Y., Ber, R., Mamroud, E., Kronman, C., Shafferman, A., Velan, B. (2003). Effective Protective Immunity to Yersinia pestis Infection Conferred by DNA Vaccine Coding for Derivatives of the F1 Capsular Antigen. Infect. Immun. 71: 374-383 [Abstract] [Full Text]
Bosma, G. C., Kim, J., Urich, T., Fath, D. M., Cotticelli, M. G., Ruetsch, N. R., Radic, M. Z., Bosma, M. J. (2002). DNA-dependent Protein Kinase Activity Is Not Required for Immunoglobulin Class Switching. J. Exp. Med. 196: 1483-1495 [Abstract] [Full Text]
Kheradmand, F., Kiss, A., Xu, J., Lee, S.-H., Kolattukudy, P. E., Corry, D. B. (2002). A Protease-Activated Pathway Underlying Th Cell Type 2 Activation and Allergic Lung Disease. J. Immunol. 169: 5904-5911 [Abstract] [Full Text]
Taneichi, M., Naito, S., Kato, H., Tanaka, Y., Mori, M., Nakano, Y., Yamamura, H., Ishida, H., Komuro, K., Uchida, T. (2002). T Cell-Independent Regulation of IgE Antibody Production Induced by Surface-Linked Liposomal Antigen. J. Immunol. 169: 4246-4252 [Abstract] [Full Text]
Laurencikiene, J., Deveikaite, V., Severinson, E. (2001). HS1,2 Enhancer Regulation of Germline {epsilon} and {gamma}2b Promoters in Murine B Lymphocytes: Evidence for Specific Promoter-Enhancer Interactions. J. Immunol. 167: 3257-3265 [Abstract] [Full Text]
Okada, H., Attanucci, J., Giezeman-Smits, K. M., Brissette-Storkus, C., Fellows, W. K., Gambotto, A., Pollack, I. F., Pogue-Geile, K., Lotze, M. T., Bozik, M. E., Chambers, W. H. (2001). Immunization with an Antigen Identified by Cytokine Tumor Vaccine-assisted SEREX (CAS) Suppressed Growth of the Rat 9L Glioma in Vivo. Cancer Res. 61: 2625-2631 [Abstract] [Full Text]
Lillard, J. W. Jr., Boyaka, P. N., Taub, D. D., McGhee, J. R. (2001). RANTES Potentiates Antigen-Specific Mucosal Immune Responses. J. Immunol. 166: 162-169 [Abstract] [Full Text]
BORISH, L. C., NELSON, H. S., LANZ, M. J., CLAUSSEN, L., WHITMORE, J. B., AGOSTI, J. M., GARRISON, L. (1999). Interleukin-4 Receptor in Moderate Atopic Asthma . A Phase I/II Randomized, Placebo-controlled Trial. Am. J. Respir. Crit. Care Med. 160: 1816-1823 [Abstract] [Full Text]
Sugie, K., Tomura, T., Takakura, K., Kawano, T., Taniguchi, M., Grey, H. M., Ishizaka, K. (1999). Target cells for an immunosuppressive cytokine, glycosylation-inhibiting factor. Int Immunol 11: 1149-1156 [Abstract] [Full Text]
Dabbagh, K., Takeyama, K., Lee, H.-M., Ueki, I. F., Lausier, J. A., Nadel, J. A. (1999). IL-4 Induces Mucin Gene Expression and Goblet Cell Metaplasia In Vitro and In Vivo. J. Immunol. 162: 6233-6237 [Abstract] [Full Text]
Lillard, J. W. Jr., Boyaka, P. N., Hedrick, J. A., Zlotnik, A., McGhee, J. R. (1999). Lymphotactin Acts as an Innate Mucosal Adjuvant. J. Immunol. 162: 1959-1965 [Abstract] [Full Text]
Lillard, J. W. Jr., Boyaka, P. N., Chertov, O., Oppenheim, J. J., McGhee, J. R. (1999). Mechanisms for induction of acquired host immunity by neutrophil peptide defensins. Proc. Natl. Acad. Sci. USA 96: 651-656 [Abstract] [Full Text]
Chiaramonte, M. G., Schopf, L. R., Neben, T. Y., Cheever, A. W., Donaldson, D. D., Wynn, T. A. (1999). IL-13 Is a Key Regulatory Cytokine for Th2 Cell-Mediated Pulmonary Granuloma Formation and IgE Responses Induced by Schistosoma mansoni Eggs. J. Immunol. 162: 920-930 [Abstract] [Full Text]
Qiu, G., Stavnezer, J. (1998). Overexpression of BSAP/Pax-5 Inhibits Switching to IgA and Enhances Switching to IgE in the I.29{micro} B Cell Line. J. Immunol. 161: 2906-2918 [Abstract] [Full Text]
Ryan, J. J., McReynolds, L. J., Huang, H., Nelms, K., Paul, W. E. (1998). Characterization of a Mobile Stat6 Activation Motif in the Human IL-4 Receptor. J. Immunol. 161: 1811-1821 [Abstract] [Full Text]
Bembridge, G. P., Lopez, J. A., Cook, R., Melero, J. A., Taylor, G. (1998). Recombinant Vaccinia Virus Coexpressing the F Protein of Respiratory Syncytial Virus (RSV) and Interleukin-4 (IL-4) Does Not Inhibit the Development of RSV-Specific Memory Cytotoxic T Lymphocytes, whereas Priming Is Diminished in the Presence of High Levels of IL-2 or Gamma Interferon. J. Virol. 72: 4080-4087 [Abstract] [Full Text]
De Sanctis, G. T., Wolyniec, W. W., Green, F. H.�Y., Qin, S., Jiao, A., Finn, P. W., Noonan, T., Joetham, A. A., Gelfand, E., Doerschuk, C. M., Drazen, J. M. (1997). Reduction of allergic airway responses in P-selectin-deficient mice. J. Appl. Physiol. 83: 681-687 [Abstract] [Full Text]
Hikida, M., Mori, M., Takai, T., Tomochika, K.-i., Hamatani, K., Ohmori, H. (1996). Reexpression of RAG-1 and RAG-2 Genes in Activated Mature Mouse B cells. Science 274: 2092-2094 [Abstract] [Full Text]
Levine, A. D., Rangwala, S. H., Peel, M. A., Leimgruber, R. M., Manning, J. A., Bishop, B. F., Olins, P. O. (1995). High Level Expression and Refolding of Mouse Interleukin 4 Synthesized in Escherichia coli. J. Biol. Chem. 270: 7445-7452 [Abstract] [Full Text]