Journal of Experimental Medicine, Vol 166, 283-288, Copyright © 1987 by Rockefeller University Press

ARTICLES

In vitro mutagenesis at a single residue introduces B and T cell epitopes into a class I HLA molecule

RD Salter, C Clayberger, CE Lomen, AM Krensky and P Parham

We have studied the interaction of HLA class I antigens with alloreactive cytotoxic T lymphocytes and monoclonal antibodies using site-directed mutagenesis and expression of an HLA-Aw68.1 gene. Two mutants containing distinct substitutions at polymorphic residues near the NH2-terminal end of the alpha 2 domain were made. One mutant with substitutions at positions 95 and 97 corresponding to residues found in HLA-A2.1 showed no alterations in binding of HLA-Aw68- or HLA-A2- specific monoclonal antibodies, but was reactive with some HLA-A2- specific CTL clones. A second mutant, in which glycine at position 107 was replaced with tryptophan found at that position in HLA-A2.1, was recognized by HLA-A2-specific CTL clones and HLA-A2, Aw69-specific monoclonal antibodies. Thus, substitution of a single amino acid residue at position 107 of the HLA-Aw68.1 molecule generates an allospecific determinant shared with HLA-A2.1 and recognized by both B and T lymphocytes.
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This article has been cited by other articles:

• Pettersen, R. D., Gaudernack, G., Olafsen, M. K., Lie, S. O., Hestdal, K. (1998). The TCR-Binding Region of the HLA Class I {alpha}2 Domain Signals Rapid Fas-Independent Cell Death: A Direct Pathway for T Cell-Mediated Killing of Target Cells?. J. Immunol. 160: 4343-4352 [Abstract] [Full Text]  

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