Journal of Experimental Medicine, Vol 165, 1453-1458, Copyright © 1987 by Rockefeller University Press

ARTICLES

Functional gamma chain-associated T cell receptors on cerebrospinal fluid-derived natural killer-like T cell clones

SL Ang, JG Seidman, GM Peterman, AD Duby, D Benjamin, SJ Lee and DA Hafler

We have derived 33 independent T cell clones from the cerebrospinal fluid (CSF) of a patient with subacute sclerosing panencephalitis using a single T cell cloning method. 6% (2 of 33) of these clones express the T cell receptor gamma (TCR-gamma) protein and are called CSF TCR- gamma clones. Phenotypic analyses of the CSF TCR-gamma clones indicate that they are WT-31-, CD3+, CD4-, and CD8-. The TCR-gamma protein exists on the cell surface as part of an 85-kD disulphide-linked dimer noncovalently associated with the CD3 polypeptides. The CSF TCR-gamma clones have NK-like activity that can be inhibited by anti-CD3 mAbs. Both CSF TCR-gamma clones proliferated in response to anti-CD3 mAbs coupled to Sepharose beads and/or IL-2. Furthermore, stimulation of one of these clones with anti-CD3 mAbs results in a rapid rise in intracellular calcium. These data suggest that T cells bearing the CD3- TCR-gamma protein complex are functional and play a role in the human immune response.
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This article has been cited by other articles:

• Hata, S, Satyanarayana, K, Devlin, P, Band, H, McLean, J, Strominger, J., Brenner, M., Krangel, M. (1988). Extensive junctional diversity of rearranged human T cell receptor delta genes. Science 240: 1541-1544 [Abstract]  

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