Prolonged survival of actively enhanced rat renal allografts despite accelerated cellular infiltration and rapid induction of both class I and class II MHC antigens

doi:10.1084/jem.165.3.891

This Article

	Full Text (PDF)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Armstrong, H. E.
	Articles by Bradley, J. A.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Armstrong, H. E.
	Articles by Bradley, J. A.


Social Bookmarking

	What's this?

ARTICLES

Prolonged survival of actively enhanced rat renal allografts despite accelerated cellular infiltration and rapid induction of both class I and class II MHC antigens

HE Armstrong, EM Bolton, I McMillan, SC Spencer and JA Bradley

Administration of 1 ml of donor whole blood 7 d before renal transplantation produces long-term (greater than 100 d) graft survival in the DA (RT1a) into PVG (RT1c) rat strain combination. Using this model, the pattern and phenotype of infiltrating leukocytes were examined in rejecting and enhanced renal allografts, at days 1, 3, 5, and 7 after transplantation, by immunohistologic techniques. Paradoxically, enhanced grafts showed a more rapid and substantial leukocyte infiltrate, the phenotype of which was similar to that in rejecting grafts except for a reduced number of MRC OX-8+ cells and MRC OX-39+ cells. Graft infiltrating cells and splenocytes from transfused animals showed similar, although modest, levels of both nonspecific cytotoxicity and alloantigen-specific cytotoxicity. Immunohistologic analysis of MHC antigen distribution within the allograft revealed, unexpectedly, that enhanced grafts underwent an accelerated and extensive induction of both donor class I and class II MHC antigens. These findings were confirmed by allospecific quantitative absorption analysis, which showed severalfold increases in class I and class II MHC antigens by day 3 in enhanced grafts but not until day 5 in rejecting grafts. An additional observation was the more rapid disappearance of donor interstitial cells from enhanced grafts. These findings emphasize the overwhelming suppressive effect induced by an organ allograft after preoperative blood transfusion despite the associated induction of large numbers of potential effector cells and increased target antigen density within the graft.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Kitade, H., Kawai, M., Rutgeerts, O., Landuyt, W., Waer, M., Mathieu, C., Pirenne, J. (2005). Early Presence of Regulatory Cells in Transplanted Rats Rendered Tolerant by Donor-Specific Blood Transfusion. J. Immunol. 175: 4963-4970 [Abstract] [Full Text]
Kataoka, M., Margenthaler, J. A., Ku, G., Flye, M. W. (2003). Development of Infectious Tolerance After Donor-Specific Transfusion and Rat Heart Transplantation. J. Immunol. 171: 204-211 [Abstract] [Full Text]
Guillet, M., Brouard, S., Gagne, K., Sebille, F., Cuturi, M.-C., Delsuc, M.-A., Soulillou, J.-P. (2002). Different Qualitative and Quantitative Regulation of V{beta} TCR Transcripts During Early Acute Allograft Rejection and Tolerance Induction. J. Immunol. 168: 5088-5095 [Abstract] [Full Text]
Yang, C.-P., Shittu, E., Bell, E. B. (2000). Specific B Cell Tolerance Is Induced by Cyclosporin A Plus Donor-Specific Blood Transfusion Pretreatment: Prolonged Survival of MHC Class I Disparate Cardiac Allografts. J. Immunol. 164: 2427-2432 [Abstract] [Full Text]
Qian, Z., Wasowska, B. A., Behrens, E., Cangello, D. L., Brody, J. R., Kadkol, S. S., Horwitz, L., Liu, J., Lowenstein, C., Hess, A. D., Sanfilippo, F., Baldwin, W. M. III (1999). C6 Produced by Macrophages Contributes to Cardiac Allograft Rejection. Am. J. Pathol. 155: 1293-1302 [Abstract] [Full Text]
ROSSINI, A. A., GREINER, D. L., MORDES, J. P. (1999). Induction of Immunologic Tolerance for Transplantation. Physiol. Rev. 79: 99-141 [Abstract] [Full Text]