Journal of Experimental Medicine, Vol 164, 661-666, Copyright © 1986 by Rockefeller University Press

ARTICLES

Induction and detection of a human endothelial activation antigen in vivo

RS Cotran, MA Gimbrone Jr, MP Bevilacqua, DL Mendrick and JS Pober

We used a murine mAb, H4/18, raised by immunization with IL-1-treated human umbilical vein endothelial cell cultures, to localize an endothelial activation antigen in induced human delayed hypersensitivity reactions (DHR) and in pathological tissues. We used streptococcus varidase to elicit DHR in human skin and we examined sequential skin biopsies with the immunoperoxidase technique. There was no staining for H4/18 binding antigen in normal endothelium of skin and other tissues; strong positive staining, localized to vascular endothelium, was seen at 16 and 23 h but disappeared by 6 d, when the DHR had faded. H4/18 binding antigen, also confined to endothelium, was detected in lymph nodes, skin, and other tissues exhibiting immune/inflammatory reactions. The studies indicate that H4/18 is a useful marker for activated endothelium in vivo and they support the relevance of in vitro studies on inducible endothelial cell functions.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Dudda, J. C., Perdue, N., Bachtanian, E., Campbell, D. J. (2008). Foxp3+ regulatory T cells maintain immune homeostasis in the skin. J. Exp. Med. 205: 1559-1565 [Abstract] [Full Text]  
• Liu, M., Kluger, M. S., D'Alessio, A., Garcia-Cardena, G., Pober, J. S. (2008). Regulation of Arterial-Venous Differences in Tumor Necrosis Factor Responsiveness of Endothelial Cells by Anatomic Context. Am. J. Pathol. 172: 1088-1099 [Abstract] [Full Text]  
• Wu, S.-Q., Aird, W. C. (2005). Thrombin, TNF-{alpha}, and LPS exert overlapping but nonidentical effects on gene expression in endothelial cells and vascular smooth muscle cells. Am. J. Physiol. Heart Circ. Physiol. 289: H873-H885 [Abstract] [Full Text]  
• Chong, B. F., Murphy, J.-E., Kupper, T. S., Fuhlbrigge, R. C. (2004). E-Selectin, Thymus- and Activation-Regulated Chemokine/CCL17, and Intercellular Adhesion Molecule-1 Are Constitutively Coexpressed in Dermal Microvessels: A Foundation for a Cutaneous Immunosurveillance System. J. Immunol. 172: 1575-1581 [Abstract] [Full Text]  
• Ishii, H., Yoshida, M., Rosenzweig, A., Gimbrone, M. A. Jr, Yasukochi, Y., Numano, F. (2000). Adenoviral transduction of human E-selectin into isolated, perfused, rat aortic segments: an ex vivo model for studying leukocyte-endothelial interactions. J. Leukoc. Biol. 68: 687-692 [Abstract] [Full Text]  
• Kawai, T., Seki, M., Watanabe, H., Eastcott, J. W., Smith, D. J., Taubman, M. A. (2000). Th1 transmigration anergy: a new concept of endothelial cell-T cell regulatory interaction. Int Immunol 12: 937-948 [Abstract] [Full Text]  
• Galea, J., Rebuck, N., Finn, A., Manche, A., Moat, N. (1998). Expression of soluble endothelial adhesion molecules in clinical cardiopulmonary bypass. Perfusion 13: 314-321 [Abstract]  
• Tietz, W., Allemand, Y., Borges, E., von Laer, D., Hallmann, R., Vestweber, D., Hamann, A. (1998). CD4+ T Cells Migrate into Inflamed Skin Only If They Express Ligands for E- and P-Selectin. J. Immunol. 161: 963-970 [Abstract] [Full Text]  
• Bhatti, M, Chapman, P, Peters, M, Haskard, D, Hodgson, H J F (1998). Visualising E-selectin in the detection and evaluation of inflammatory bowel disease. Gut 43: 40-47 [Abstract] [Full Text]  
• Cines, D. B., Pollak, E. S., Buck, C. A., Loscalzo, J., Zimmerman, G. A., McEver, R. P., Pober, J. S., Wick, T. M., Konkle, B. A., Schwartz, B. S., Barnathan, E. S., McCrae, K. R., Hug, B. A., Schmidt, A.-M., Stern, D. M. (1998). Endothelial Cells in Physiology and in the Pathophysiology of Vascular Disorders. Blood 91: 3527-3561 [Full Text]  
• Spragg, D. D., Alford, D. R., Greferath, R., Larsen, C. E., Lee, K.-D., Gurtner, G. C., Cybulsky, M. I., Tosi, P. F., Nicolau, C., Gimbrone, M. A. Jr. (1997). Immunotargeting of liposomes to activated vascular endothelial cells: A strategy for site-selective delivery in the cardiovascular system. Proc. Natl. Acad. Sci. USA 94: 8795-8800 [Abstract] [Full Text]  
• Wayne Smith, C. (1997). Potential Significance of Circulating E-Selectin. Circulation 95: 1986-1988 [Full Text]  
• Courtney, J. M, Matata, B. M, Yin, H. Q, Esposito, A., Mahiout, A., Taggart, D. P, Lowe, G. (1996). The influence of biomaterials on inflammatory responses to cardiopulmonary bypass. Perfusion 11: 220-228  
• Weerwind, P. W., Maessen, J. G., van Tits, L. J. H., Stad, R. K., Fransen, E. J., de Jong, D. S., Penn, O. C. K. M. (1995). INFLUENCE OF DURAFLO II HEPARIN-TREATED EXTRACORPOREAL CIRCUITS ON THE SYSTEMIC INFLAMMATORY RESPONSE IN PATIENTS HAVING CORONARY BYPASS. J. Thorac. Cardiovasc. Surg. 110: 1633-1641 [Abstract] [Full Text]  
• Salmi, M, Jalkanen, S (1992). A 90-kilodalton endothelial cell molecule mediating lymphocyte binding in humans. Science 257: 1407-1409 [Abstract]  
• Cybulsky, M., Gimbrone, M. Jr (1991). Endothelial expression of a mononuclear leukocyte adhesion molecule during atherogenesis. Science 251: 788-791 [Abstract]  
• Phillips, M., Nudelman, E, Gaeta, F., Perez, M, Singhal, A., Hakomori, S, Paulson, J. (1990). ELAM-1 mediates cell adhesion by recognition of a carbohydrate ligand, sialyl-Lex. Science 250: 1130-1132 [Abstract]  
• Rice, G., Bevilacqua, M. (1989). An inducible endothelial cell surface glycoprotein mediates melanoma adhesion. Science 246: 1303-1306 [Abstract]  
• Bevilacqua, M., Stengelin, S, Gimbrone, M. Jr, Seed, B (1989). Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins and lectins. Science 243: 1160-1165 [Abstract]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS