Journal of Experimental Medicine, Vol 163, 1518-1528, Copyright © 1986 by Rockefeller University Press

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Mapping of a second recombination hot spot within the I-E region of the mouse H-2 gene complex

WP Lafuse, N Berg, S Savarirayan and CS David

The crossover points of nine intra-I region recombinant mouse strains were determined by restriction fragment analysis. The recombinants were examined for the presence of k and p haplotype specific DNA restriction endonuclease sites. These restriction sites were a Sac I site between the E beta and E beta 2 genes, a Hpa I site within the E beta 2 gene, and a Rsa I site approximately 1 kb to the right of the E alpha gene. Seven of the recombinants were found to have crossovers between the Hpa I and the Rsa I site. This analysis suggests that a recombination hot spot exists within this segment. This segment is approximately 12-14 kb, and contains the E alpha gene and the intervening sequence between the E beta 2 and E alpha genes.
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This article has been cited by other articles:

• Qin, J., Richardson, L. L., Jasin, M., Handel, M. A., Arnheim, N. (2004). Mouse Strains with an Active H2-Ea Meiotic Recombination Hot Spot Exhibit Increased Levels of H2-Ea-Specific DNA Breaks in Testicular Germ Cells. Mol. Cell. Biol. 24: 1655-1666 [Abstract] [Full Text]  
• Khambata, S, Mody, J, Modzelewski, A, Heine, D, Passmore, H C (1996). Ea recombinational hot spot in the mouse major histocompatibility complex maps to the fourth intron of the Ea gene.. Genome Res 6: 195-201 [Abstract]  
• Kobori, J., Strauss, E, Minard, K, Hood, L (1986). Molecular analysis of the hotspot of recombination in the murine major histocompatibility complex. Science 234: 173-179 [Abstract]  

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