Crosslinking of surface immunoglobulin and Fc receptors on B lymphocytes inhibits stimulation of inositol phospholipid breakdown via the antigen receptors

doi:10.1084/jem.162.6.1825

This Article

	Full Text (PDF)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Bijsterbosch, M. K.
	Articles by Klaus, G. G.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Bijsterbosch, M. K.
	Articles by Klaus, G. G.


Social Bookmarking

	What's this?

ARTICLES

Crosslinking of surface immunoglobulin and Fc receptors on B lymphocytes inhibits stimulation of inositol phospholipid breakdown via the antigen receptors

MK Bijsterbosch and GG Klaus

F(ab')2 fragments of rabbit anti-mouse Ig induce proliferation of murine B lymphocytes, whereas the intact antibodies are not mitogenic. F(ab')2 anti-Ig stimulates the rapid breakdown of inositol phospholipids in B cells, resulting in the prolonged release of inositol (poly)phosphates and diacylglycerol. In marked contrast, intact anti-Ig initially induces a comparable response, which is abrogated after some 30 s. Blocking either the Fc receptors on the B cells or the Fc portion of the antibodies significantly reversed the inhibitory effect. On the other hand, both forms of anti-Ig elicited comparable increases in free cytoplasmic Ca2+ levels in B cells. These results therefore indicate that crosslinkage of Fc and surface Ig receptors on B cells inhibits inositol phospholipid breakdown (but not Ca2+ flux) resulting from ligation of the antigen receptors. Since there is evidence implicating inositol phospholipid breakdown in the induction of cell growth, this effect could provide a biochemical explanation for the known capacity of antigen-antibody complexes to inhibit B cell activation.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Getahun, A., Dahlstrom, J., Wernersson, S., Heyman, B. (2004). IgG2a-Mediated Enhancement of Antibody and T Cell Responses and Its Relation to Inhibitory and Activating Fc{gamma} Receptors. J. Immunol. 172: 5269-5276 [Abstract] [Full Text]
Karlsson, M. C. I., Getahun, A., Heyman, B. (2001). Fc{gamma}RIIB in IgG-Mediated Suppression of Antibody Responses: Different Impact In Vivo and In Vitro. J. Immunol. 167: 5558-5564 [Abstract] [Full Text]
Fong, D. C., Brauweiler, A., Minskoff, S. A., Bruhns, P., Tamir, I., Mellman, I., Daeron, M., Cambier, J. C. (2000). Mutational Analysis Reveals Multiple Distinct Sites Within Fc{gamma} Receptor IIB That Function in Inhibitory Signaling. J. Immunol. 165: 4453-4462 [Abstract] [Full Text]
Terness, P., Navolan, D., Opelz, G., Roelcke, D. (1999). Inverse Association Between IgG-Anti-kappa and Antierythrocyte Autoantibodies in Patients With Cold Agglutination. Blood 94: 4343-4346 [Abstract] [Full Text]
Tanguay, D., Pavlovic, S., Piatelli, M. J., Bartek, J., Chiles, T. C. (1999). B Cell Antigen Receptor-Mediated Activation of Cyclin-Dependent Retinoblastoma Protein Kinases and Inhibition by Co-Cross-Linking with Fc{gamma} Receptors. J. Immunol. 163: 3160-3168 [Abstract] [Full Text]
Gupta, N., Scharenberg, A. M., Fruman, D. A., Cantley, L. C., Kinet, J.-P., Long, E. O. (1999). The SH2 Domain-containing Inositol 5'-Phosphatase (SHIP) Recruits the p85 Subunit of Phosphoinositide 3-Kinase during Fcgamma RIIb1-mediated Inhibition of B Cell Receptor Signaling. J. Biol. Chem. 274: 7489-7494 [Abstract] [Full Text]
Karlsson, M. C.�I., Wernersson, S., Diaz de Stahl, T., Gustavsson, S., Heyman, B. (1999). Efficient IgG-mediated suppression of primary antibody responses in Fcgamma receptor-deficient mice. Proc. Natl. Acad. Sci. USA 96: 2244-2249 [Abstract] [Full Text]
Minskoff, S. A., Matter, K., Mellman, I. (1998). Cutting Edge: Fc{gamma}RII-B1 Regulates the Presentation of B Cell Receptor-Bound Antigens. J. Immunol. 161: 2079-2083 [Abstract] [Full Text]
Hunter, S., Indik, Z. K., Kim, M.-K., Cauley, M. D., Park, J.-G., Schreiber, A. D. (1998). Inhibition of Fcgamma Receptor-Mediated Phagocytosis by a Nonphagocytic Fcgamma Receptor. Blood 91: 1762-1768 [Abstract] [Full Text]
Malbec, O., Fong, D. C., Turner, M., Tybulewicz, V. L. J., Cambier, J. C., Fridman, W. H., Daeron, M. (1998). Fc{epsilon} Receptor I-Associated lyn-Dependent Phosphorylation of Fc{gamma} Receptor IIB During Negative Regulation of Mast Cell Activation. J. Immunol. 160: 1647-1658 [Abstract] [Full Text]
Buhl, A. M., Pleiman, C. M., Rickert, R. C., Cambier, J. C. (1997). Qualitative Regulation of B Cell Antigen Receptor Signaling by CD19: Selective Requirement for PI3-Kinase Activation, Inositol-1,4,5-Trisphosphate Production and Ca2+ Mobilization. J. Exp. Med. 186: 1897-1910 [Abstract] [Full Text]
Vuica, M., Desiderio, S., Schneck, J. P. (1997). Differential Effects of B Cell Receptor and B Cell Receptor-Fcgamma RIIB1 Engagement on Docking of Csk to GTPase-activating Protein (GAP)-associated p62. J. Exp. Med. 186: 259-267 [Abstract] [Full Text]
Welschof, M., Terness, P., Kipriyanov, S. M., Stanescu, D., Breitling, F., Dorsam, H., Dubel, S., Little, M., Opelz, G. (1997). The antigen-binding domain of a human IgG-anti-F(ab')2�autoantibody. Proc. Natl. Acad. Sci. USA 94: 1902-1907 [Abstract] [Full Text]
Sarkar, S., Schlottmann, K., Cooney, D., Coggeshall, K. M. (1996). Negative Signaling via Fcgamma RIIB1 in B Cells Blocks Phospholipase Cgamma 2 Tyrosine Phosphorylation but Not Syk or Lyn Activation. J. Biol. Chem. 271: 20182-20186 [Abstract] [Full Text]
DeFranco, A., Law, D. (1995). Tyrosine phosphatases and the antibody response. Science 268: 263-264
D'Ambrosio, D, Hippen, K., Minskoff, S., Mellman, I, Pani, G, Siminovitch, K., Cambier, J. (1995). Recruitment and activation of PTP1C in negative regulation of antigen receptor signaling by Fc gamma RIIB1. Science 268: 293-297 [Abstract]