Purification and partial biochemical characterization of normal human interleukin 1

doi:10.1084/jem.160.3.772

This Article

	Full Text (PDF)
	Alert me when this article is cited

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Schmidt, J. A.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Schmidt, J. A.


Social Bookmarking

	What's this?

ARTICLES

Purification and partial biochemical characterization of normal human interleukin 1

JA Schmidt

A protocol for the rapid, efficient purification of the major charged species of human interleukin 1 (IL-1) has been developed using high performance anion exchange and size exclusion chromatography. The isolated material is pure as determined by sodium dodecyl sulfate (SDS) gradient polyacrylamide gel electrophoresis (PAGE) and analytical isoelectric focusing (IEF). The molecular weight of the purified material is 15,000 and the isoelectric point (pI) is 6.8, values that are in good agreement with those previously reported for human IL-1. 10(-10) M concentrations of the purified material give half-maximal stimulation in the thymocyte proliferation assay. Amounts of IL-1 sufficient for receptor studies and detailed biochemical analysis can now be produced on a regular basis.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Schonbeck, U., Mach, F., Libby, P. (1998). Generation of Biologically Active IL-1{beta} by Matrix Metalloproteinases: A Novel Caspase-1-Independent Pathway of IL-1{beta} Processing. J. Immunol. 161: 3340-3346 [Abstract] [Full Text]
Nordan, R., Potter, M (1986). A macrophage-derived factor required by plasmacytomas for survival and proliferation in vitro. Science 233: 566-569 [Abstract]
Milanese, C, Richardson, N., Reinherz, E. (1986). Identification of a T helper cell-derived lymphokine that activates resting T lymphocytes. Science 231: 1118-1122 [Abstract]