Identification of a strain-specific malarial antigen exposed on the surface of Plasmodium falciparum-infected erythrocytes

doi:10.1084/jem.159.6.1567

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Identification of a strain-specific malarial antigen exposed on the surface of Plasmodium falciparum-infected erythrocytes

JH Leech, JW Barnwell, LH Miller and RJ Howard

We have identified strain-specific antigens with Camp and St. Lucia strains of P. falciparum of Mr approximately 285,000 and approximately 260,000, respectively. These strain-specific antigens were metabolically labeled with radioactive amino acids, indicating that they were of parasite origin rather than altered host components. These proteins had the properties of a molecule exposed on the surface of infected erythrocytes (IE). First, the proteins are accessible to lactoperoxidase-catalyzed radioiodination of IE. Second, the radioiodinated proteins were cleaved by low concentrations of trypsin (0.1 microgram/ml). Third, these antigens were immunoprecipitated after addition of immune sera to intact IE. Fourth, the strain-specific immuno-precipitation of these proteins correlated with the capacity of immune sera to block cytoadherence of IE in a strain-specific fashion. Fifth, the strain-specific antigen had detergent solubility properties (i.e., insolubility in 1% Triton X-100, solubility in 5% sodium dodecyl sulfate) similar to the variant antigen of P. knowlesi, which has been proven to be a malarial protein exposed on the erythrocyte surface.
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Pettersson, F., Vogt, A. M., Jonsson, C., Mok, B. W., Shamaei-Tousi, A., Bergstrom, S., Chen, Q., Wahlgren, M. (2005). Whole-Body Imaging of Sequestration of Plasmodium falciparum in the Rat. Infect. Immun. 73: 7736-7746 [Abstract] [Full Text]
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Janssen, C. S., Phillips, R. S., Turner, C. M. R., Barrett, M. P. (2004). Plasmodium interspersed repeats: the major multigene superfamily of malaria parasites. Nucleic Acids Res 32: 5712-5720 [Abstract] [Full Text]
Mahanty, S., Saul, A., Miller, L. H. (2003). Progress in the development of recombinant and synthetic blood-stage malaria vaccines. J. Exp. Biol. 206: 3781-3788 [Abstract] [Full Text]
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Piper, K. P., Hayward, R. E., Cox, M. J., Day, K. P. (1999). Malaria Transmission and Naturally Acquired Immunity to PfEMP-1. Infect. Immun. 67: 6369-6374 [Abstract] [Full Text]
Boubou, M. I., Collette, A., Voegtle, D., Mazier, D., Cazenave, P.-A., Pied, S. (1999). T cell response in malaria pathogenesis: selective increase in T cells carrying the TCR V{beta}8 during experimental cerebral malaria. Int Immunol 11: 1553-1562 [Abstract] [Full Text]
Waller, K. L., Cooke, B. M., Nunomura, W., Mohandas, N., Coppel, R. L. (1999). Mapping the Binding Domains Involved in the Interaction between the Plasmodium falciparum Knob-associated Histidine-rich Protein (KAHRP) and the Cytoadherence Ligand P. falciparum Erythrocyte Membrane Protein 1 (PfEMP1). J. Biol. Chem. 274: 23808-23813 [Abstract] [Full Text]
Kyes, S. A., Rowe, J. A., Kriek, N., Newbold, C. I. (1999). Rifins: A second family of clonally variant proteins expressed on the surface of red cells infected with Plasmodium falciparum. Proc. Natl. Acad. Sci. USA 96: 9333-9338 [Abstract] [Full Text]
Reeder, J. C., Cowman, A. F., Davern, K. M., Beeson, J. G., Thompson, J. K., Rogerson, S. J., Brown, G. V. (1999). The adhesion of Plasmodium falciparum-infected erythrocytes to chondroitin sulfate A is mediated by P. falciparum erythrocyte membrane protein 1. Proc. Natl. Acad. Sci. USA 96: 5198-5202 [Abstract] [Full Text]
Hayward, R. E., Tiwari, B., Piper, K. P., Baruch, D. I., Day, K. P. (1999). Virulence and transmission success of the malarial parasite Plasmodium falciparum. Proc. Natl. Acad. Sci. USA 96: 4563-4568 [Abstract] [Full Text]
Beeson, J. G., Chai, W., Rogerson, S. J., Lawson, A. M., Brown, G. V. (1998). Inhibition of Binding of Malaria-Infected Erythrocytes by a Tetradecasaccharide Fraction from Chondroitin Sulfate A. Infect. Immun. 66: 3397-3402 [Abstract] [Full Text]
Echaide, I. E., Hines, S. A., McElwain, T. F., Suarez, C. E., McGuire, T. C., Palmer, G. H. (1998). In Vivo Binding of Immunoglobulin M to the Surfaces of Babesia bigemina-Infected Erythrocytes. Infect. Immun. 66: 2922-2927 [Abstract] [Full Text]
Baruch, D. I., Ma, X. C., Singh, H. B., Bi, X., Pasloske, B. L., Howard, R. J. (1997). Identification of a Region of PfEMP1 That Mediates Adherence of Plasmodium falciparum Infected Erythrocytes to CD36: Conserved Function With Variant Sequence. Blood 90: 3766-3775 [Abstract] [Full Text]
Hadley, T. J., Peiper, S. C. (1997). From Malaria to Chemokine Receptor: The Emerging Physiologic Role of the Duffy Blood Group Antigen. Blood 89: 3077-3091 [Full Text]
Enderle, Th., Ha, T., Ogletree, D.�F., Chemla, D.�S., Magowan, C., Weiss, S. (1997). Membrane specific mapping and colocalization of malarial and host skeletal proteins in the Plasmodium falciparum infected erythrocyte by dual-color near-field scanning optical�microscopy. Proc. Natl. Acad. Sci. USA 94: 520-525 [Abstract] [Full Text]
Miller, L., Howard, R., Carter, R, Good, M., Nussenzweig, V, Nussenzweig, R. (1986). Research toward malaria vaccines. Science 234: 1349-1356 [Abstract]
Marsh, K, Howard, R. (1986). Antigens induced on erythrocytes by P. falciparum: expression of diverse and conserved determinants. Science 231: 150-153 [Abstract]
Trenholme, K. R., Gardiner, D. L., Holt, D. C., Thomas, E. A., Cowman, A. F., Kemp, D. J. (2000). clag9: A cytoadherence gene in Plasmodium falciparum essential for binding of parasitized erythrocytes to CD36. Proc. Natl. Acad. Sci. USA 97: 4029-4033 [Abstract] [Full Text]