The major histocompatibility complex-restricted antigen receptor on T cells. II. Role of the L3T4 product

doi:10.1084/jem.158.4.1077

3rd Skeletal Biology and Medicine Symposium

This Article

	Full Text (PDF)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Marrack, P.
	Articles by Kappler, J.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Marrack, P.
	Articles by Kappler, J.


Social Bookmarking

	What's this?

ARTICLES

The major histocompatibility complex-restricted antigen receptor on T cells. II. Role of the L3T4 product

P Marrack, R Endres, R Shimonkevitz, A Zlotnik, D Dialynas, F Fitch and J Kappler

We have examined the role of the murine homologue of Leu-3 T4, L3T4, in recognition of antigen in association with products of the major histocompatibility complex (Ag/MHC) by murine T cell hybridomas. A series of ovalbumin (OVA)/I-Ad-specific T cell hybridomas were ranked in their sensitivity to Ag/I by measuring their ability to respond to low doses of OVA, or their sensitivity to inhibition by anti-I-Ad antibodies. T cell hybridomas with low apparent avidity for OVA/I-Ad, i.e. that did not respond well to low concentrations of OVA and were easily inhibited by anti-I-Ad, were also easily inhibited by anti-L3T4 antibodies. The reverse was true for T cell hybridomas with apparent high avidity for Ag/MHC. We found that the presence of low doses of anti-L3T4 antibodies caused T cell hybridomas to respond less well to low doses of Ag, and to be more easily inhibited by anti-I-Ad antibodies. These results suggested that the role of the L3T4 molecule is to increase the overall avidity of the reaction between T cells and Ag-presenting cells. In support of this idea was the discovery of several L3T4- subclones of one of our L3T4+ T cell hybridomas, D0.11.10. The L3T4- subclones had the same amount of receptor for OVA/I- Ad as their L3T4+ parent, as detected by an anti-receptor monoclonal antibody. The L3T4- subclones, however, responded less well to low doses of OVA, and were more easily inhibited by anti-I-Ad antibodies than their L3T4/ parent. These results showed that the L3T4 molecule was not required for surface expression of, or functional activity of, the T cell receptor for Ag/MHC. The L3T4 molecule did, however, increase the sensitivity with which the T cell reacted with Ag/MHC on Ag-presenting cells.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Thedrez, A., de Lalla, C., Allain, S., Zaccagnino, L., Sidobre, S., Garavaglia, C., Borsellino, G., Dellabona, P., Bonneville, M., Scotet, E., Casorati, G. (2007). CD4 engagement by CD1d potentiates activation of CD4+ invariant NKT cells. Blood 110: 251-258 [Abstract] [Full Text]
Moldovan, M.-C., Sabbagh, L., Breton, G., Sekaly, R.-P., Krummel, M. F. (2006). Triggering of T Cell Activation via CD4 Dimers. J. Immunol. 176: 5438-5445 [Abstract] [Full Text]
Wang, Q., Strong, J., Killeen, N. (2001). Homeostatic Competition Among T Cells Revealed by Conditional Inactivation of the Mouse Cd4 Gene. J. Exp. Med. 194: 1721-1730 [Abstract] [Full Text]
Wang, Q., Malherbe, L., Zhang, D., Zingler, K., Glaichenhaus, N., Killeen, N. (2001). CD4 Promotes Breadth in the TCR Repertoire. J. Immunol. 167: 4311-4320 [Abstract] [Full Text]
Strong, J., Wang, Q., Killeen, N. (2001). Impaired survival of T helper cells in the absence of CD4. Proc. Natl. Acad. Sci. USA 10.1073/pnas.051329698v1 [Abstract] [Full Text]
Krummel, M. F., Sjaastad, M. D., Wülfing, C., Davis, M. M. (2000). Differential Clustering of CD4 and CD3zeta During T Cell Recognition. Science 289: 1349-1352 [Abstract] [Full Text]
Wang, R., Wang-Zhu, Y., Gabaglia, C. R., Kimachi, K., Grey, H. M. (1999). The Stimulation of Low-Affinity, Nontolerized Clones by Heteroclitic Antigen Analogues Causes the Breaking of Tolerance Established to an Immunodominant T Cell Epitope. J. Exp. Med. 190: 983-994 [Abstract] [Full Text]
Thatte, J., Qadri, A., Radu, C., Ward, E. S. (1999). Molecular Requirements for T Cell Recognition by a Major Histocompatibility Complex Class II-restricted T Cell Receptor: The Involvement of the Fourth Hypervariable Loop of the Valpha Domain. J. Exp. Med. 189: 509-520 [Abstract] [Full Text]
Mostaghel, E. A., Riberdy, J. M., Steeber, D. A., Doyle, C. (1998). Coreceptor-Independent T Cell Activation in Mice Expressing MHC Class II Molecules Mutated in the CD4 Binding Domain. J. Immunol. 161: 6559-6566 [Abstract] [Full Text]
Hamad, A. R. A., O'Herrin, S. M., Lebowitz, M. S., Srikrishnan, A., Bieler, J., Schneck, J., Pardoll, D. (1998). Potent T Cell Activation with Dimeric Peptide-Major Histocompatibility Complex Class II Ligand: The Role of CD4 Coreceptor. J. Exp. Med. 188: 1633-1640 [Abstract] [Full Text]
HERITAGE, P.�L., UNDERDOWN, B.�J., ARSENAULT, A.�L., SNIDER, D.�P., MCDERMOTT, M.�R. (1997). Comparison of Murine Nasal-associated Lymphoid Tissue and Peyer's Patches. Am. J. Respir. Crit. Care Med. 156: 1256-1262 [Abstract] [Full Text]
Zhu, Y., Lambert, K., Corless, C., Copeland, N. G., Gilbert, D. J., Jenkins, N. A., D'Andrea, A. D. (1997). DUB-2 Is a Member of a Novel Family of Cytokine-inducible Deubiquitinating Enzymes. J. Biol. Chem. 272: 51-57 [Abstract] [Full Text]
Lenert, P, Kroon, D, Spiegelberg, H, Golub, E., Zanetti, M (1990). Human CD4 binds immunoglobulins. Science 248: 1639-1643 [Abstract]
Schwartz, R. (1990). A cell culture model for T lymphocyte clonal anergy. Science 248: 1349-1356 [Abstract]
Shizuru, J., Gregory, A., Chao, C., Fathman, C. (1987). Islet allograft survival after a single course of treatment of recipient with antibody to L3T4. Science 237: 278-280 [Abstract]
Waldor, M., Sriram, S, Hardy, R, Herzenberg, L., Herzenberg, L., Lanier, L, Lim, M, Steinman, L (1985). Reversal of experimental allergic encephalomyelitis with monoclonal antibody to a T-cell subset marker. Science 227: 415-417 [Abstract]
Amdjadi, K., Sefton, B. M. (2000). Ultraviolet Light-induced Stimulation of the JNK Mitogen-activated Protein Kinase in the Absence of Src Family Tyrosine Kinase Activation. J. Biol. Chem. 275: 22520-22525 [Abstract] [Full Text]
Strong, J., Wang, Q., Killeen, N. (2001). Impaired survival of T helper cells in the absence of CD4. Proc. Natl. Acad. Sci. USA 98: 2566-2571 [Abstract] [Full Text]