Journal of Experimental Medicine, Vol 158, 1061-1076, Copyright © 1983 by Rockefeller University Press
Epitopes on H-2Dd somatic cell mutants recognized by cytotoxic T cells
TA Potter, MA Palladino, DB Wilson and TV Rajan
We have generated several cell lines that express an altered H-2Dd
molecule. These cell lines, which were selected for by the failure to
express the serological specificity reacting with the monoclonal antibody
34-2-12, have also undergone alterations in epitopes recognized by CTL. One
of the mutants, 2.12(-4) was not killed by an allogeneic anti-Dd CTL line,
CTLL-A2, even though this line was cytotoxic for the parental cell line and
two other 34-2-12- mutant lines. Two of the 34-2-12- mutant lines had an
identical serological profile using other monoclonal Dd antibodies, however
these two mutants differed markedly in their susceptibility to cytotoxicity
by CTLL-A2. In addition to the determinants recognized by allogeneic CTL we
also examined the effect of the mutation on the determinants involved in
restricting the anti-FITC modified-self-cytotoxic response. An anti-
FITC-Dd CTL line did not react with two of the mutants and reacted only
weakly with the other mutant, demonstrating not only that the Dd epitopes
recognized by this cell line and the allogeneic CTL were different, but
also that it is possible for a H-2 class I molecule to express epitopes
recognized by allogeneic CTL but not epitopes that function as restricting
elements to certain antigens. The observation that both T cell- and B
cell-defined determinants were altered in these mutant cell lines is in
contrast to the findings, with the mutant mouse strains which were selected
for by changes in T cell-defined determinants, which show few, if any,
alterations to serological specificities. Characterization of T
cell-recognized epitopes expressed on serologically selected somatic cell
variants may therefore prove to be most useful for the study of
structure-function relationships of H-2 class I molecules.
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