Journal of Experimental Medicine, Vol 157, 2002-2016, Copyright © 1983 by Rockefeller University Press
IR gene regulation of the response to trinitrophenyl-polysaccharides. Two independent genes are required for antibody production
LM Hillstrom and JE Niederhuber
The primary in vitro antibody response to TNP-Ficoll was found to be under
H-2-restricted Ir gene control. Strains B10(H-2b), B10.A(H-2a), and
B10.S(9R) (H-2t4) were consistently low responders while strains
D2.GD(H-2g2), B10.GD(H-2g2), and B10.S(H-2s) were high responders. The in
vitro TNP-Ficoll response in congenic recombinant and F1 hybrid mice
demonstrated the requirement for complementation of two independent Ir
genes. One Ir gene mapped in or to the left of the I-A subregion with high
responder alleles being s or d. The second Ir gene mapped to the right of
the I-E subregion and required b or s alleles for complementation. These
results were further supported by the ability to block the TNP-Ficoll
response by appropriate anti-Ia serum pretreatment of the
antigen-presenting macrophages. When a structurally different
polysaccharide antigen TNP-dextran was used, an identical pattern of
restriction was observed.
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