The Journal of Experimental Medicine
Janeway's Immunobiology 7th Edition
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Journal of Experimental Medicine, Vol 157, 1064-1069, Copyright © 1983 by Rockefeller University Press


ARTICLES

Polymorphonuclear neutrophils express the common acute lymphoblastic leukemia antigen

J Cossman, LM Neckers, WJ Leonard and WC Greene

Monoclonal antibodies J5, VIL-A1, and BA-3, known to react with the common acute lymphoblastic leukemia antigen (CALLA) were found to specifically stain normal human polymorphonuclear neutrophils (PMN). The antigen detected on PMN had a molecular weight (95,000-110,000 mol wt) close to that of CALLA (95,000-100,000 mol wt) and thus these surface membrane antigens are likely related, if not identical. The fluorescent staining intensity of PMN is comparable to that of CALLA- positive leukemic cells and the presence of PMN in patient samples could potentially produce false-positive results in diagnosis.
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