Journal of Experimental Medicine, Vol 157, 473-485, Copyright © 1983 by Rockefeller University Press
Human monocytes are associated with the formation of fibrin
N Hogg
Purified populations of human monocytes (greater than 99% monocytic)
contain a subpopulation of cells (8-20%) that will surround themselves with
a radial array of needles within 15 s after exposure to plasma. The needles
are composed of fibrin as demonstrated by gel electrophoresis of isolated
needles and by their reaction with a monoclonal antibody, UC45, that is
specific for the alpha chain of fibrin. The addition of UC45 antibody to
plasma inhibits the formation of the fibrin needles by monocytes,
suggesting that the recognized antigen is important for polymerization. In
contrast, UC45 binds weakly to conventional plasma fibrin and does not
prevent clotting of plasma although the process is prolonged. Thus, the
UC45 antigen is more prominently represented or more accessible on monocyte
fibrin. At least 1% of the monocytes were able to form fibers at the
earliest time that they could be tested. A greater proportion developed
this ability between 2 and 6 h in culture. Macrophages from lung, thymus,
and breast milk, when incubated for similar periods of time, do not form
fibrin needles. However, phagocytes from tonsils were extremely active in
this respect. It is speculated that monocytes may be induced to express
fibrin as part of their activities in inflammatory lesions.
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