Journal of Experimental Medicine, Vol 156, 703-718, Copyright © 1982 by Rockefeller University Press
Products of the IgT-C region of chromosome 12 are maturational markers for T cells. Sequence of appearance in immunocompetent T cells parallels ontogenetic appearance of Tthyd, Tindd, and Tsud
FL Owen
Monoclonal antibodies specific for three T cell alloantigens linked to the
immunoglobulin complex on chromosome 12 were used to establish the order of
expression of these antigens on immunocompetent cells and in ontogeny.
Modification of monoclonal antibodies with fluorescein isothiocyanate
(FITC) and use with anti-FITC and complement has amplified lytic capacity
of the monoclonals and allowed us to complete a distribution study of Tsud,
Tindd, And Tthyd alloantigens on immunocompetent cells. Tthyd is expressed
on both cortisone-sensitive and cortisone-resistant thymocytes; Tsud and
Tindd are on "mature" cortisone-resistant cells. Tthyd is also expressed on
a Thy-1.2-bearing recirculating marrow cell but is undetectable in the
peripheral T cell pool. In contrast, resting spleen and lymph node T cells
express Tsud and Tindd; antigen-activated populations express these two
cells in high frequencies. These antigens must be markers for relatively
differentiated cells because "nude" animals, which have pre-T cells, fail
to express these determinants. All three antigens segregate independently
in our T cell hybrids, arising from adult peripheral node cells, supporting
the hypothesis that these are three separate structural products of a gene
complex. In contrast, fetal T cell hybrids fail to express these antigens.
The appearance of all three antigens on the cell surface in ontogeny is
postnatal; Tthyd is expressed at days 1-2, Tindd at days 2-3, and Tsud at
days 5-6. If the T cell isotype genes are organized similar to the
immunoglobulin loci, then the parallels in maturational expression on
immunocompetent cells and in ontogeny would lead one to speculate a gene
order of Tthyd leads to Tindd leads to Tsud. Orientation with respect to
the centromere is unknown.
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