The Journal of Experimental Medicine
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Journal of Experimental Medicine, Vol 156, 652-657, Copyright © 1982 by Rockefeller University Press


ARTICLES

Demonstration of a third structurally distinct human Ia beta chain by two-dimensional gel electrophoresis

RW Karr, CC Kannapell, JA Stein, TC Fuller, RJ Duquesnoy, GE Rodey, DL Mann, HM Gebel and BD Schwartz

Previous studies have indicated that HLA-DR homozygous cell lines express two Ia alpha and Ia beta chains that combine to form at least two Ia molecules. This report demonstrates by two-dimensional gel electrophoresis the existence of a third structurally distinct human Ia beta chain on DR2 and DR5 cell lines. This suggests that at least five separate genes control the expression of Ia molecules on HLA-DR homozygous cell lines.
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