GD3, a prominent ganglioside of human melanoma. Detection and characterisation by mouse monoclonal antibody

doi:10.1084/jem.155.4.1133

This Article

	Full Text (PDF)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Pukel, C. S.
	Articles by Old, L. J.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Pukel, C. S.
	Articles by Old, L. J.


Social Bookmarking

	What's this?

ARTICLES

GD3, a prominent ganglioside of human melanoma. Detection and characterisation by mouse monoclonal antibody

CS Pukel, KO Lloyd, LR Travassos, WG Dippold, HF Oettgen and LJ Old

Mouse monoclonal antibody AbR24 has a high degree of specificity for human melanoma cells when tested on viable cultured cells using the protein A mixed hemagglutinin serological assay. The antigen detected by this antibody has been isolated from melanoma cells and shown to be GD3 ganglioside by compositional and partial structural analysis and by comparison with authentic GD3 in thin layer chromatography (TLC). AbR24 reacts with authentic GD3, but not with any other ganglioside tested. Using TLC and reactivity with AbR24, a wide range of cells and tissues was examined for the presence of GD3. A new serological assay, termed glycolipid-mediated immune adherence, was devised for assaying the reactivity of AbR24 with gangliosides. Melanomas (cultured cells or tumor tissue) were shown to have GD3 and GM3 as major gangliosides. Other cells and tissues examined also contained GD3, but usually only in low amounts. Melanomas (and MOLT-4, a T cell line) were characterized by a simplified ganglioside profile with GD3 and GM3 as major components. The apparent discrepancy between the ubiquitous presence of GD3 and the serological specificity of AbR24 for melanoma cells can be explained in terms of localization and concentration of GD3 in different cells.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Attrill, H., Imamura, A., Sharma, R. S., Kiso, M., Crocker, P. R., van Aalten, D. M. F. (2006). Siglec-7 Undergoes a Major Conformational Change When Complexed with the {alpha}(2,8)-Disialylganglioside GT1b. J. Biol. Chem. 281: 32774-32783 [Abstract] [Full Text]
Moon, S.-K., Kang, S.-K., Kim, C.-H. (2006). Reactive oxygen species mediates disialoganglioside GD3-induced inhibition of ERK1/2 and matrix metalloproteinase-9 expression in vascular smooth muscle cells. FASEB J. 20: 1387-1395 [Abstract] [Full Text]
Chen, H. Y., Challa, A. K., Varki, A. (2006). 9-O-Acetylation of Exogenously Added Ganglioside GD3: THE GD3 MOLECULE INDUCES ITS OWN O-ACETYLATION MACHINERY. J. Biol. Chem. 281: 7825-7833 [Abstract] [Full Text]
Hamamura, K., Furukawa, K., Hayashi, T., Hattori, T., Nakano, J., Nakashima, H., Okuda, T., Mizutani, H., Hattori, H., Ueda, M., Urano, T., Lloyd, K. O., Furukawa, K. (2005). Ganglioside GD3 promotes cell growth and invasion through p130Cas and paxillin in malignant melanoma cells. Proc. Natl. Acad. Sci. USA 102: 11041-11046 [Abstract] [Full Text]
Yamaji, T., Mitsuki, M., Teranishi, T., Hashimoto, Y. (2005). Characterization of inhibitory signaling motifs of the natural killer cell receptor Siglec-7: attenuated recruitment of phosphatases by the receptor is attributed to two amino acids in the motifs. Glycobiology 15: 667-676 [Abstract] [Full Text]
Moon, S.-K., Kim, H.-M., Lee, Y.-C., Kim, C.-H. (2004). Disialoganglioside (GD3) Synthase Gene Expression Suppresses Vascular Smooth Muscle Cell Responses via the Inhibition of ERK1/2 Phosphorylation, Cell Cycle Progression, and Matrix Metalloproteinase-9 Expression. J. Biol. Chem. 279: 33063-33070 [Abstract] [Full Text]
Wu, D. Y., Segal, N. H., Sidobre, S., Kronenberg, M., Chapman, P. B. (2003). Cross-presentation of Disialoganglioside GD3 to Natural Killer T Cells. J. Exp. Med. 198: 173-181 [Abstract] [Full Text]
Satake, H., Chen, H. Y., Varki, A. (2003). Genes Modulated by Expression of GD3 Synthase in Chinese Hamster Ovary Cells. EVIDENCE THAT THE Tis21 GENE IS INVOLVED IN THE INDUCTION OF GD3 9-O-ACETYLATION. J. Biol. Chem. 278: 7942-7948 [Abstract] [Full Text]
Garcia-Ruiz, C., Colell, A., Morales, A., Calvo, M., Enrich, C., Fernandez-Checa, J. C. (2002). Trafficking of Ganglioside GD3 to Mitochondria by Tumor Necrosis Factor-alpha. J. Biol. Chem. 277: 36443-36448 [Abstract] [Full Text]
Sonnenburg, J. L., van Halbeek, H., Varki, A. (2002). Characterization of the Acid Stability of Glycosidically Linked Neuraminic Acid. USE IN DETECTING DE-N-ACETYL-GANGLIOSIDES IN HUMAN MELANOMA. J. Biol. Chem. 277: 17502-17510 [Abstract] [Full Text]
Bhunia, A. K., Schwarzmann, G., Chatterjee, S. (2002). GD3 Recruits Reactive Oxygen Species to Induce Cell Proliferation and Apoptosis in Human Aortic Smooth Muscle Cells. J. Biol. Chem. 277: 16396-16402 [Abstract] [Full Text]
Yamaji, T., Teranishi, T., Alphey, M. S., Crocker, P. R., Hashimoto, Y. (2002). A Small Region of the Natural Killer Cell Receptor, Siglec-7, Is Responsible for Its Preferred Binding to alpha 2,8-Disialyl and Branched alpha 2,6-Sialyl Residues. A COMPARISON WITH Siglec-9. J. Biol. Chem. 277: 6324-6332 [Abstract] [Full Text]
Scott, A. M., Lee, F.-T., Hopkins, W., Cebon, J. S., Wheatley, J. M., Liu, Z., Smyth, F. E., Murone, C., Sturrock, S., MacGregor, D., Hanai, N., Inoue, K., Yamasaki, M., Brechbiel, M. W., Davis, I. D., Murphy, R., Hannah, A., Lim-Joon, M., Chan, T., Chong, G., Ritter, G., Hoffman, E. W., Burgess, A. W., Old, L. J. (2001). Specific Targeting, Biodistribution, and Lack of Immunogenicity of Chimeric Anti-GD3 Monoclonal Antibody KM871 in Patients With Metastatic Melanoma: Results of a Phase I Trial. JCO 19: 3976-3987 [Abstract] [Full Text]
Lee, F.-T., Rigopoulos, A., Hall, C., Clarke, K., Cody, S. H., Smyth, F. E., Liu, Z., Brechbiel, M. W., Hanai, N., Nice, E. C., Catimel, B., Burgess, A. W., Welt, S., Ritter, G., Old, L. J., Scott, A. M. (2001). Specific Localization, Gamma Camera Imaging, and Intracellular Trafficking of Radiolabelled Chimeric Anti-GD3 Ganglioside Monoclonal Antibody KM871 in SK-MEL-28 Melanoma Xenografts. Cancer Res. 61: 4474-4482 [Abstract] [Full Text]
Kambe, N., Kambe, M., Kochan, J. P., Schwartz, L. B. (2001). Human skin-derived mast cells can proliferate while retaining their characteristic functional and protease phenotypes. Blood 97: 2045-2052 [Abstract] [Full Text]
Fukumoto, S., Mutoh, T., Hasegawa, T., Miyazaki, H., Okada, M., Goto, G., Furukawa, K., Urano, T., Furukawa, K. (2000). GD3 Synthase Gene Expression in PC12 Cells Results in the Continuous Activation of TrkA and ERK1/2 and Enhanced Proliferation. J. Biol. Chem. 275: 5832-5838 [Abstract] [Full Text]
Chammas, R., Sonnenburg, J. L., Watson, N. E., Tai, T., Farquhar, M. G., Varki, N. M., Varki, A. (1999). De-N-acetyl-gangliosides in Humans: Unusual Subcellular Distribution of a Novel Tumor Antigen. Cancer Res. 59: 1337-1346 [Abstract] [Full Text]
Kaminski, M. J., MacKenzie, C. R., Mooibroek, M. J., Dahms, T. E.�S., Hirama, T., Houghton, A. N., Chapman, P. B., Evans, S. V. (1999). The Role of Homophilic Binding in Anti-tumor Antibody R24 Recognition of Molecular Surfaces. DEMONSTRATION OF AN INTERMOLECULAR beta -SHEET INTERACTION BETWEEN VH DOMAINS. J. Biol. Chem. 274: 5597-5604 [Abstract] [Full Text]
Schwarz, A., Futerman, A. H. (1997). Determination of the Localization of Gangliosides Using Anti-ganglioside Antibodies: Comparison of Fixation Methods. J. Histochem. Cytochem. 45: 611-618 [Abstract] [Full Text]
Kanamori, A., Nakayama, J., Fukuda, M. N., Stallcup, W. B., Sasaki, K., Fukuda, M., Hirabayashi, Y. (1997). Expression cloning and characterization of a cDNA encoding a novel membrane protein required for the formation of O-acetylated ganglioside: A putative acetyl-CoA�transporter. Proc. Natl. Acad. Sci. USA 94: 2897-2902 [Abstract] [Full Text]
Nakayama, J., Fukuda, M. N., Hirabayashi, Y., Kanamori, A., Sasaki, K., Nishi, T., Fukuda, M. (1996). Expression Cloning of a Human G[IMAGE] Synthase. J. Biol. Chem. 271: 3684-3691 [Abstract] [Full Text]
Gard, A., Burrell, M., Pfeiffer, S., Rudge, J., Williams, W. (1995). Astroglial control of oligodendrocyte survival mediated by PDGF and leukemia inhibitory factor-like protein. Development 121: 2187-2197 [Abstract]
Cheresh, D., Reisfeld, R., Varki, A. (1984). O-acetylation of disialoganglioside GD3 by human melanoma cells creates a unique antigenic determinant. Science 225: 844-846 [Abstract]
Nudelman, E, Kannagi, R, Hakomori, S, Parsons, M, Lipinski, M, Wiels, J, Fellous, M, Tursz, T (1983). A glycolipid antigen associated with Burkitt lymphoma defined by a monoclonal antibody. Science 220: 509-511 [Abstract]