Journal of Experimental Medicine, Vol 154, 1570-1583, Copyright © 1981 by Rockefeller University Press

ARTICLES

Regulation of immune responses by I-J gene products. I. Production and characterization of anti-I-J monoclonal antibodies

C Waltenbaugh

Using a novel, two-step functional screening procedure, we have isolated hybridoma B cell lines secreting monoclonal antibodies directed against gene products of the I-Jb and I-Jk subregions of the mouse H-2 complex. These monoclonal antibodies act in vitro by allowing nonresponder spleen cells to respond to normally suppressive quantities of poly(Glu50Tyr50) (GT) (WF8 series of anti-I-Jk monoclonal antibodies) or to suboptimal concentration of poly(Glu60Ala30Tyr10) (WF9 series of anti-I-Jb monoclonal antibodies). Some of the culture supernates that show augmenting activity bind GT-specific T cell- derived suppressor factor (GT-TsF), indicating that some monoclonal antiantibodies display a nonspecific enhancing effect, or, more likely, that anti-I-J monoclonal antibodies have been produced against I-J determinants not found on TsF. It is this last possibility that is most intriguing and that might serve as a means for exploring the heterogeneity of the I-J subregion. It is also possible that some of our monoclonal anti-I-J antibodies might detect antigenic determinants selectively expressed on suppressor T cells, helper T cells, and/or macrophages. In addition, we have demonstrated that monoclonal anti-I-J antibodies should be useful in the biochemical characterization and purification of a monoclonal GT-TsF. These haplotype-specific anti-I-J monoclonal antibodies should prove to be powerful tools for future studies exploring the role of I-J gene products in the regulation of specific immune responses.
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This article has been cited by other articles:

• Granstein, R., Parrish, J., McAuliffe, D., Waltenbaugh, C, Greene, M. (1984). Immunologic inhibition of ultraviolet radiation-induced tumor suppressor cell activity. Science 224: 615-617 [Abstract]  
• Hayes, C., Klyczek, K., Krum, D., Whitcomb, R., Hullett, D., Cantor, H (1984). Chromosome 4 Jt gene controls murine T cell surface I-J expression. Science 223: 559-563 [Abstract]  

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