The Journal of Experimental Medicine
Keystone Symposia
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Journal of Experimental Medicine, Vol 154, 978-982, Copyright © 1981 by Rockefeller University Press

ARTICLES

Responder T cells depleted of alloreactive cells react to antigen presented on allogeneic macrophages from nonresponder strains

N Ishii, CN Baxevanis, ZA Nagy and J Klein

T cells from strains responder to the antigen poly(Glu40 Ala60) (GA) were depleted of alloreactive cells by bromo-deoxyuridine and light treatment, and were subsequently primed in vitro in GA presented by allogeneic macrophages from nonresponder strains. Antigen-specific secondary proliferative responses restricted by allogeneic Ia molecules of the macrophages were obtained in all strain combinations tested. These data indicate that Ir gene-controlled nonresponsiveness cannot be the result of a failure of antigen presentation.
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