Immunogenic variants obtained by mutagenesis of mouse mastocytoma P815. II. T lymphocyte-mediated cytolysis

doi:10.1084/jem.152.5.1184

This Article

	Full Text (PDF)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Boon, T.
	Articles by Marchand, M.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Boon, T.
	Articles by Marchand, M.


Social Bookmarking

	What's this?

ARTICLES

Immunogenic variants obtained by mutagenesis of mouse mastocytoma P815. II. T lymphocyte-mediated cytolysis

T Boon, J Van Snick, A Van Pel, C Uyttenhove and M Marchand

Tumor cell variants that were rejected by syngeneic mice (tum-) were obtained from mastocytoma P815 by mutagenesis (as described in the accompanying report (13). A considerable T lymphocyte-mediated lysis was observed upon incubation of these tum- variants with peritoneal exudate cells collected a few days after an intraperitoneal challenge of immune animals. Spleen cells from these animals were cytolytic after stimulation in vitro with the immunizing variant. New antigens, absent from the original P815 tum+ cells, were detected on 15 of the 21 tum- variants that were tested. All these antigens appeared to be different. No new antigen was detected on any of 10 mutagenized P815 clones that had retained their ability to form tumors. We compared the evidence obtained in vivo and in vitro for the presence of specific antigens on five tum- variants. Three variants were shown both in vivo and in vitro to carry an individual antigen. One showed no specificity either in vivo or in vitro. However, for one variant, no specificity was observed in vivo, although cytolysis tests demonstrated the existence of a singular antigenic specificity.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Carrasco, J., Van Pel, A., Neyns, B., Lethe, B., Brasseur, F., Renkvist, N., van der Bruggen, P., van Baren, N., Paulus, R., Thielemans, K., Boon, T., Godelaine, D. (2008). Vaccination of a Melanoma Patient with Mature Dendritic Cells Pulsed with MAGE-3 Peptides Triggers the Activity of Nonvaccine Anti-Tumor Cells. J. Immunol. 180: 3585-3593 [Abstract] [Full Text]
Zoeteweij, J. P., Epperson, D. E., Porter, J. D., Zhang, C. X., Frolova, O. Y., Constantinides, A. P., Fuhrmann, S. R., El-Amine, M., Tian, J.-H., Ellingsworth, L. R., Glenn, G. M. (2006). GM1 Binding-Deficient Exotoxin Is a Potent Noninflammatory Broad Spectrum Intradermal Immunoadjuvant. J. Immunol. 177: 1197-1207 [Abstract] [Full Text]
Godelaine, D., Carrasco, J., Lucas, S., Karanikas, V., Schuler-Thurner, B., Coulie, P. G., Schuler, G., Boon, T., Van Pel, A. (2003). Polyclonal CTL Responses Observed in Melanoma Patients Vaccinated with Dendritic Cells Pulsed with a MAGE-3.A1 Peptide. J. Immunol. 171: 4893-4897 [Abstract] [Full Text]
Heidecker, L., Brasseur, F., Probst-Kepper, M., Gueguen, M., Boon, T., Van den Eynde, B. J. (2000). Cytolytic T Lymphocytes Raised Against a Human Bladder Carcinoma Recognize an Antigen Encoded by Gene MAGE-A12. J. Immunol. 164: 6041-6045 [Abstract] [Full Text]
van Baren, N., Brasseur, F., Godelaine, D., Hames, G., Ferrant, A., Lehmann, F., Andre, M., Ravoet, C., Doyen, C., Spagnoli, G. C., Bakkus, M., Thielemans, K., Boon, T. (1999). Genes Encoding Tumor-Specific Antigens Are Expressed in Human Myeloma Cells. Blood 94: 1156-1164 [Abstract] [Full Text]
De Backer, O., Arden, K. C., Boretti, M., Vantomme, V., De Smet, C., Czekay, S., Viars, C. S., De Plaen, E., Brasseur, F., Chomez, P., Van den Eynde, B., Boon, T., van der Bruggen, P. (1999). Characterization of the GAGE Genes That Are Expressed in Various Human Cancers and in Normal Testis. Cancer Res. 59: 3157-3165 [Abstract] [Full Text]
Dobrzanski, M. J., Reome, J. B., Dutton, R. W. (1999). Therapeutic Effects of Tumor-Reactive Type 1 and Type 2 CD8+ T Cell Subpopulations in Established Pulmonary Metastases. J. Immunol. 162: 6671-6680 [Abstract] [Full Text]
Huang, L.-Q., Brasseur, F., Serrano, A., De Plaen, E., van der Bruggen, P., Boon, T., Van Pel, A. (1999). Cytolytic T Lymphocytes Recognize an Antigen Encoded by MAGE-A10 on a Human Melanoma. J. Immunol. 162: 6849-6854 [Abstract] [Full Text]
Bilsborough, J., Van Pel, A., Uyttenhove, C., Boon, T., Van den Eynde, B. J. (1999). Identification of a Second Major Tumor-Specific Antigen Recognized by CTLs on Mouse Mastocytoma P815. J. Immunol. 162: 3534-3540 [Abstract] [Full Text]
Gueguen, M., Patard, J.-J., Gaugler, B., Brasseur, F., Renauld, J.-C., Van Cangh, P. J., Boon, T., Van den Eynde, B. J. (1998). An Antigen Recognized by Autologous CTLs on a Human Bladder Carcinoma. J. Immunol. 160: 6188-6194 [Abstract] [Full Text]
Mandruzzato, S., Brasseur, F., Andry, G., Boon, T., Bruggen, P. v. d. (1997). A CASP-8 Mutation Recognized by Cytolytic T Lymphocytes on a Human Head and Neck Carcinoma. J. Exp. Med. 186: 785-793 [Abstract] [Full Text]
van der Bruggen, P, Traversari, C, Chomez, P, Lurquin, C, De Plaen, E, Van den Eynde, B, Knuth, A, Boon, T (1991). A gene encoding an antigen recognized by cytolytic T lymphocytes on a human melanoma. Science 254: 1643-1647 [Abstract]