Immunogenic variants obtained by mutagenesis of mouse mastocytoma P815. I. Rejection by syngeneic mice

doi:10.1084/jem.152.5.1175

This Article

	Full Text (PDF)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Uyttenhove, C.
	Articles by Boon, T.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Uyttenhove, C.
	Articles by Boon, T.


Social Bookmarking

	What's this?

ARTICLES

Immunogenic variants obtained by mutagenesis of mouse mastocytoma P815. I. Rejection by syngeneic mice

C Uyttenhove, J Van Snick and T Boon

We have reported that it is possible to obtain variants that are incapable of forming progressive tumour in syngeneic mice (tum-) by mutagenesis and cloning of a teratocarcinoma and a Lewis lung carcinoma cell line. These observations were extended to the ascitic P815 mastocytoma of mouse strain DB/2. After a treatment with the mutagen N- methyl-N"-nitro-N-nitrosoguanidine, we obtained a high frequency (14%) of tum- clones. A colony assay indicated that after a period of rapid multiplication extending to approximately 10 d after injection, the P815 tum- cells were rejected by a process that was usually completed by day 15. No rejection was observed in sublethally irradiated animals. The immunological nature of the rejection of the P815 variants was further inferred because, upon rejection, the mice acquired a radioresistant specific protection that could be transferred adoptively with spleen cells. Cross-immunization patterns demonstrated the presence of singular antigenic specificities on three of the five variants that were examined. In addition, a common antigen was found on all the tum- variants and the original cells that were capable of forming progressive tumors in syngeneic mice (tum+). Mice injected with tum- cells were significantly protected against a tum+ challenge, even though no significant protection was generated by irradiated tum+ cells. A study of the T lymphocyte-mediated cytolysis against the P815 variants described here is presented in the accompanying report (9).
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Aublin, A., Ciofani, M., Willkomm, N., Hamrouni, A., Szymczak-Workman, A. L., Takahashi, T., Sandjeu, Y., Guillaume, P., Vignali, D. A. A., Michielin, O., Zuniga-Pflucker, J. C., Maryanski, J. L. (2006). A Natural Structural Variant of the Mouse TCR beta-Chain Displays Intrinsic Receptor Function and Antigen Specificity. J. Immunol. 177: 8587-8594 [Abstract] [Full Text]
Liu, Y., Bi, X., Xu, S., Xiang, J. (2005). Tumor-Infiltrating Dendritic Cell Subsets of Progressive or Regressive Tumors Induce Suppressive or Protective Immune Responses. Cancer Res. 65: 4955-4962 [Abstract] [Full Text]
Wang, J., Shaw, J. L., Mullen, C. A. (2002). Down-Regulation of Antihost Alloreactivity after Bone Marrow Transplant Permits Relapse of Hematological Malignancy. Cancer Res. 62: 208-212 [Abstract] [Full Text]
Iwashiro, M., Messer, R. J., Peterson, K. E., Stromnes, I. M., Sugie, T., Hasenkrug, K. J. (2001). Immunosuppression by CD4+ regulatory T cells induced by chronic retroviral infection. Proc. Natl. Acad. Sci. USA 10.1073/pnas.151174198v1 [Abstract] [Full Text]
Sturmhoefel, K., Lee, K., Gray, G. S., Thomas, J., Zollner, R., O'Toole, M., Swiniarski, H., Dorner, A., Wolf, S. F. (1999). Potent Activity of Soluble B7-IgG Fusion Proteins in Therapy of Established Tumors and as Vaccine Adjuvant. Cancer Res. 59: 4964-4972 [Abstract] [Full Text]
Bilsborough, J., Van Pel, A., Uyttenhove, C., Boon, T., Van den Eynde, B. J. (1999). Identification of a Second Major Tumor-Specific Antigen Recognized by CTLs on Mouse Mastocytoma P815. J. Immunol. 162: 3534-3540 [Abstract] [Full Text]
Livingston, B. D., Alexander, J., Crimi, C., Oseroff, C., Celis, E., Daly, K., Guidotti, L. G., Chisari, F. V., Fikes, J., Chesnut, R. W., Sette, A. (1999). Altered Helper T Lymphocyte Function Associated with Chronic Hepatitis B Virus Infection and its Role in Response to Therapeutic Vaccination in Humans. J. Immunol. 162: 3088-3095 [Abstract] [Full Text]
Iwashiro, M., Messer, R. J., Peterson, K. E., Stromnes, I. M., Sugie, T., Hasenkrug, K. J. (2001). Immunosuppression by CD4+ regulatory T cells induced by chronic retroviral infection. Proc. Natl. Acad. Sci. USA 98: 9226-9230 [Abstract] [Full Text]