Journal of Experimental Medicine, Vol 152, 336-349, Copyright © 1980 by Rockefeller University Press
Role of the major histocompatibility gene products in regulating the antibody response to dinitrophenylated poly(L-Glu55,L-Ala35,L-Phe9)n
SK Pierce, NR Klinman, PH Maurer and CF Merryman
These studies were carried out to investigate the potential helper T cell
repertoire specific for the random copolymer poly(L-Glu55,L-Ala35,
L-Phe9)n(GL phi 9) of responder, nonresponder, and (responder x
nonresponder)F1 murine strains. We tested the ability of these T cells to
collaborate with dinitrophenyl (DNP)-specific primary and secondary B
lymphocytes of each strain in response to the antigen CNP-GL phi 9 in the
splenic-fragment culture system. The results of these experiments show that
there are GL phi 9-specific T lymphocytes in the responder, nonresponder,
and F1 strains; but that these three GL phi 9-specific T cell populations
differ in their collaborative potential. Responder T cells are able to
collaborate with their own syngeneic responder B cells as well as the
allogeneic nonresponder B cells in a syngeneic fashion. The F1 T cell
population resembles that of the nonresponder in its ability to collaborate
with only responder B cells in a syngeneic fashion. Analysis carried out
using appropriately selected mouse strains indicate that these results are
unlikely to be a result of positive or negative allogeneic effects. The
results obtained suggest that individuals within a given murine strain do
possess the capacity to collaborate in a syngeneic fashion with B cells of
any other MHC- allogeneic strain as well as their own MHC-identical B
cells. The nonresponder status in the response to GL phi 9 appears to be
the result of a deletion of T cells capable of recognizing antigen in the
context of B cells of the nonresponder haplotype. Thus, the MHC gene
products appear to play a determinative role in shaping the expressed
helper T cell specificity repertoire within an individual mouse strain.
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