Failure to trigger the oxidative metabolic burst by normal macrophages: possible mechanism for survival of intracellular pathogens

doi:10.1084/jem.151.2.328

This Article

	Full Text (PDF)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Wilson, C. B.
	Articles by Remington, J. S.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Wilson, C. B.
	Articles by Remington, J. S.


Social Bookmarking

	What's this?

ARTICLES

Failure to trigger the oxidative metabolic burst by normal macrophages: possible mechanism for survival of intracellular pathogens

CB Wilson, V Tsai and JS Remington

As previously reported, normal human monocytes (11) and activated mouse macrophages (9) are able to kill or inhibit intracellular replication of Toxoplasma that are not antibody coated, whereas normal human and mouse macrophages are not (7, 9). Each of these types of mononuclear phagocytes is able to kill antibody-coated Toxoplasma. In our studies, phagocytosis of antibody-coated Toxoplasma stimulated the respiratory burst by each of these types of mononuclear phagocytes, whereas phagocytosis of organisms that were not antibody coated stimulated the respiratory burst only by human monocytes and by activated mouse macrophages. Phagocytosis of Toxoplasma did not inhibit production of reactive oxygen metabolites by normal macrophages; rather, it failed to stimulate their production. Killing of Toxoplasma by monocytes from a child with X-linked chronic granulomatous disease and his heterozygote mother was impaired. Thus, reactive oxygen metabolites, perhaps in conjunction with lysosomal contents, appear to be first-line mechanisms whereby mononuclear phagocytes kill this organism. We were not able to determine the exact mechanisms whereby mononuclear phagocytes inhibit the replication of those Toxoplasma that were not killed, although both oxygen-dependent and other nonlysosomal mechanisms may be involved. The differences we observed in oxidative response to phagocytosis of Toxoplasma appear to be one determinant of the antimicrobial activity of these cells and may account for the ability of some intracellular pathogens to survive within phagocytes. These differences may be membrane related. Further studies of Toxoplasma membranes, phagocyte membrane receptors for Toxoplasma, and membrane-related mechanisms for activation of the respiratory burst are needed to define their true basis.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Lahiri, A., Das, P., Chakravortty, D. (2008). The LysR-type transcriptional regulator Hrg counteracts phagocyte oxidative burst and imparts survival advantage to Salmonella enterica serovar Typhimurium. Microbiology 154: 2837-2846 [Abstract] [Full Text]
Hajishengallis, G., Wang, M., Harokopakis, E., Triantafilou, M., Triantafilou, K. (2006). Porphyromonas gingivalis Fimbriae Proactively Modulate {beta}2 Integrin Adhesive Activity and Promote Binding to and Internalization by Macrophages.. Infect. Immun. 74: 5658-5666 [Abstract] [Full Text]
Rao, P. S. S., Lim, T. M., Leung, K. Y. (2001). Opsonized Virulent Edwardsiella tarda Strains Are Able To Adhere to and Survive and Replicate within Fish Phagocytes but Fail To Stimulate Reactive Oxygen Intermediates. Infect. Immun. 69: 5689-5697 [Abstract] [Full Text]
Kim, T.-S., Jung, Y., Na, B.-K., Kim, K.-S., Chung, P.-R. (2000). Molecular Cloning and Expression of Cu/Zn-Containing Superoxide Dismutase from Fasciola hepatica. Infect. Immun. 68: 3941-3948 [Abstract] [Full Text]
Kang, H., Remington, J. S., Suzuki, Y. (2000). Decreased Resistance of B Cell-Deficient Mice to Infection with Toxoplasma gondii Despite Unimpaired Expression of IFN-{gamma}, TNF-{alpha}, and Inducible Nitric Oxide Synthase. J. Immunol. 164: 2629-2634 [Abstract] [Full Text]
Rongrungruang, Y., Levitz, S. M. (1999). Interactions of Penicillium marneffei with Human Leukocytes In Vitro. Infect. Immun. 67: 4732-4736 [Abstract] [Full Text]
Schluter, D., Deckert-Schluter, M., Lorenz, E., Meyer, T., Rollinghoff, M., Bogdan, C. (1999). Inhibition of Inducible Nitric Oxide Synthase Exacerbates Chronic Cerebral Toxoplasmosis in Toxoplasma gondii-Susceptible C57BL/6 Mice But Does Not Reactivate the Latent Disease in T. gondii-Resistant BALB/c Mice. J. Immunol. 162: 3512-3518 [Abstract] [Full Text]
Gasser, A., Most, J. (1999). Generation of Multinucleated Giant Cells In Vitro by Culture of Human Monocytes with Mycobacterium bovis BCG in Combination with Cytokine-Containing Supernatants. Infect. Immun. 67: 395-402 [Abstract] [Full Text]
Abu Kwaik, Y., Venkataraman, C., Harb, O. S., Gao, L.-Y. (1998). Signal Transduction in the Protozoan Host Hartmannella vermiformis upon Attachment and Invasion by Legionella micdadei. Appl. Environ. Microbiol. 64: 3134-3139 [Abstract] [Full Text]
Nasarre, C., Krahenbuhl, J. L., Klei, T. R. (1998). Down Regulation of Macrophage Activation in Brugia pahangi-Infected Jirds (Meriones unguiculatus). Infect. Immun. 66: 1063-1069 [Abstract] [Full Text]
Scharton-Kersten, T. M., Yap, G., Magram, J., Sher, A. (1997). Inducible Nitric Oxide Is Essential for Host Control of Persistent but Not Acute Infection with the Intracellular Pathogen Toxoplasma gondii. J. Exp. Med. 185: 1261-1274 [Abstract] [Full Text]
Most, J., Spotl, L., Mayr, G., Gasser, A., Sarti, A., Dierich, M. P. (1997). Formation of Multinucleated Giant Cells In Vitro Is Dependent on the Stage of Monocyte to Macrophage Maturation. Blood 89: 662-671 [Abstract] [Full Text]
Morisaki, J., Heuser, J., Sibley, L. (1995). Invasion of Toxoplasma gondii occurs by active penetration of the host cell. J. Cell Sci. 108: 2457-2464 [Abstract]
Joiner, K., Fuhrman, S., Miettinen, H., Kasper, L., Mellman, I (1990). Toxoplasma gondii: fusion competence of parasitophorous vacuoles in Fc receptor-transfected fibroblasts. Science 249: 641-646 [Abstract]