Journal of Experimental Medicine, Vol 151, 1-11, Copyright © 1980 by Rockefeller University Press
Regulation of Fc fragment-induced murine spleen cell proliferation
EL Morgan and WO Weigle
Murine splenic lymphocytes proliferate in response to supernatant material
derived from Fc fragment-pulsed splenic adherent cells. The stimulatory
supernatant results from the interaction of Fc fragments with adherent
cells or adherent cell supernate. Isolation of the stimulatory material in
the supernate by Sephadex chromatography revealed that the mitogenic
component was a cleavage product of Fc with a mol wt of approximately
14,000. The spleen cell type responsible for the generation of mitogenic Fc
subfragments appears to be a macrophage. Unstimulated macrophages release
an active supernate without being exposed to Fc fragments. The supernate of
unstimulated macrophages apparently contain an enzyme which is capable of
cleaving Fc fragments into the 14,000-mol wt mitogenic molecules. The
spleen cell population induced to proliferate in response to the adherent
cell supernate is present in T-cell depleted and Sephadex G-10 filtered
cell preparations. Depletion of cells bearing immunoglobulin on their
surfaces results in a reduced proliferative response to the mitogenic
supernatant material indicating that it is probably a B cell.
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