Journal of Experimental Medicine, Vol 149, 1371-1378, Copyright © 1979 by Rockefeller University Press
Mechanisms of idiotype suppression. I. In vitro generation of idiotype- specific suppressor T cells by anti-idiotype antibodies and specific antigen
BS Kim
Normal BALB/c spleen cells are unresponsive in vitro to the
phosphorylcholine (PC) determinant in the presence of anti-idiotype
antibodies specific for the TEPC-15 myeloma protein (T15) which carries an
idiotypic determinant indistinguishable from that of most anti-PC
antibodies in BALB/c mice. The possibility that idiotype-specific
suppressor cells may be generated during the culture period was examined by
coculturing the cells with untreated syngeneic spleen cells. Cells that had
been preincubated with anti-T15 idiotype (anti- T15id) antibodies and a
PC-containing antigen, R36a for 3 d, were capable of specifically
suppressing the anti-PC response of fresh normal spleen cells, indicating
that idiotype-specific suppressor cells were generated during the culture
period. The presence of specific antigen also appeared to be necessary
because anti-T15id antibodies and a control antigen, DNP-Lys-Ficoll, were
not capable of generating such suppressor cells. Suppressor cells were
induced only in the population of spleen cells nonadherent to nylon wool
and the suppressive activity was abrogated by treatment with anti-Thy 1.2
serum and complement. These results indicate that anti-idiotype antibodies
and specific antigen can generate idiotype-specific suppressor T cells in
vitro. These in vitro results may reflect in vivo mechanisms of idiotype
suppression.
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