The Journal of Experimental Medicine
Avanti Polar Lipids
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Journal of Experimental Medicine, Vol 148, 1612-1619, Copyright © 1978 by Rockefeller University Press

ARTICLES

B-cell precursors specific to sheep erythrocytes. Estimation of frequency in a specific helper assay

MH Schreier

A sensitive, specific, and reproducible in vitro helper assay is described which is suited to limiting dilution analysis of murine B cells. 1 in about 3,000 syngeneic splenic B cells can be induced to form plaque-forming cells (PFC) to sheep erythrocytes in this system. The induction of PFC is absolutely dependent on antigen and specific helper T cells.
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