Journal of Experimental Medicine, Vol 147, 1355-1362, Copyright © 1978 by Rockefeller University Press
Differential effects of polyadenylic: polyuridylic acid and lipopolysaccharide on the generation of cytotoxic T lymphocytes
PR Narayanan and G Sundharadas
In a mixed leukocyte culture (MLC) reaction of allogenic mouse spleen cells
differing for H-2K or H-2D, only a weak cytotoxic response is generated.
This cytotoxic response is augmented significantly if bacterial
lipopolysaccharide (LPS), 5 microgram/ml, or polyadenylic acid (poly
A):polyuridylic acid (poly U), 20 microgram/ml, is present in the culture.
The cytotoxic cells generated in the presence of these two agents are
specific for sensitizing H-2K or H-2D antigen. Two lines of evidence
suggest that these two agents exert their effect at different steps in the
development of cytotoxic lymphocytes: (a) the effect of poly A:U depends on
the presence of adherent cells, whereas the effect of LPS is independent of
the presence of adherent cells and (b) LPS promotes the development of
cytotoxic cells when ultraviolet light-treated stimulating cells are used
in the MLC whereas poly A:U does not.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
