Culture of mononuclear phagocytes on a teflon surface to prevent adherence

doi:10.1084/jem.147.1.271

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Culture of mononuclear phagocytes on a teflon surface to prevent adherence

JW van der Meer, D Bulterman, TL van Zwet, I Elzenga-Claasen and R van Furth

A method is described for the culture of mononuclear phagocytes in suspension by incubation on a Teflon film to which the cells do not adhere. The characteristics of peritoneal macrophages, bone marrow mononuclear phagocytes, macrophage cell lines, and fibroblasts cultured in this way are similar to those observed after culture on glass or plastic surfaces. Culture of mononuclear phagocytes in Teflon film dishes has three important advantages: the cells can be easily harvested without damage, recovery is almost complete, and the cells are not functionally impaired. Thus, this method makes it possible to use cultured mononuclear phagocytes for many studied that could previously only be done in freshly collected cells.
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