The Journal of Experimental Medicine
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Journal of Experimental Medicine, Vol 146, 1182-1194, Copyright © 1977 by Rockefeller University Press

ARTICLES

Type I Escherichia coli pili: characterization of binding to monkey kidney cells

IE Salit and EC Gotschlich

We have demonstrated binding of purified pili from a strain of Escherichia coli to Vero cell monolayers as a model of prokaryotic- eukaryotic cell adherence. Pili bound to the tissue culture in a rapid reaction that did not require enzymatic activation. Attachment occurred optimally at pH 4-5 and could be inhibited by analogues of D-mannose, anti-pili antibodies, or by preincubation of tissue cells with mannose- specific plant lectins. Binding remained after treatment of the monolayer with glycosidases, trypsin, or a protease mixture but was enhanced after neuraminidase treatment. These results indicate that bacterial binding can occur via pili which act like lectins and presumably bind to mannose-containing glycoproteins on mammalian cell surfaces.
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