The Journal of Experimental Medicine
Cytokines in immune regulation
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Journal of Experimental Medicine, Vol 145, 693-708, Copyright © 1977 by Rockefeller University Press

ARTICLES

Generation of T-helper cells in vitro. II. Analysis of supernates derived from T-helper cell cultures

JS McDougal and DS Gordon
Parasitology Division, Center for Disease Control, Atlanta, Georgia 30333.

Supernates derived from in vitro generated T-helper cells have been analyzed for their capacity to substitute for T-cell carrier reactivity. T-helper cell supernates stimulate both a carrier-specific and nonspecific anti-DNP-PFC response to DNP-carrier conjugates in cultures of hapten-primed spleen cells. The carrier-specific and nonspecific activity can be distinguished by dosage optimum, antigen requirements, binding specificity for carrier, and in the requirement for additional splenic adherent accessory cell involvement. The active factors produced in this system are heat labile and sensitive to trypsin and periodate. They are removed by absorption with alloantisera directed toward the strain from which the supernate was derived but not by a variety of anti-immunoglobulin sera.
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