The Journal of Experimental Medicine
Cytokines Montreal 2008
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Journal of Experimental Medicine, Vol 144, 1381-1385, Copyright © 1976 by Rockefeller University Press

ARTICLES

Fractionation of human T lymphocytes on wheat germ agglutinin-sepharose

U Hellstrom, ML Dillner, S Hammarstrom and P Perlmann

T cells from human peripheral blood was purified by fractionation on columns charged with human immunoglobulin and rabbit anti-human immuno- globulin. When assayed with 125I- or fluorescein isothiocyanate-labeled wheat-germ agglutinin (WGA), a weakly binding and a strongly binding subpopulation could be distinguished. These T-cell subpopulations were fractionated on columns charged with WGA, convalently bound to Sepharose 6MB. The cells responding to the mitogens leukoagglutinin from Phaseolus vulgaris and concanavalin A were enriched in the strongly binding subpopulation (approximately 20% of the T cells) while they were depleted from the weakly binding subpopulation.
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