The Journal of Experimental Medicine
StemCell Technologies
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Journal of Experimental Medicine, Vol 143, 1265-1270, Copyright © 1976 by Rockefeller University Press

ARTICLES

Growth of B-lymphocytes clones in semisolid culture is mitogen dependent

PW Kincade, P Ralph and MA Moore

A substance which was mitogenic for murine B lymphocytes in the presence of 2-mercaptoethanol was isolated from agar. Stimulating activity of this material was stable to proteolysis or protein denaturants but was destroyed by periodate treatment. Agar-derived mitogen stimulation was distinct from that obtained with dextran sulfate or lipopolysaccharide and may define different populations of B lymphocytes.
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