Journal of Experimental Medicine, Vol 143, 1111-1121, Copyright © 1976 by Rockefeller University Press
The requirement for C3 receptors on the precursors of 19S and 7S antibody-forming cells
DW Mason
The main conclusion from this study is that C3 receptors are not required
for the generation from B cells of a thymus-dependent 7S antibody response.
The requirement for C3 receptors on the precursors of antibody-forming
cells was studied in an adoptive transfer system using thoracic duct
lymphocytes (TDL) from primed rats as a source of precursors and irradiated
recipients as hosts. 7S precursors were found in both the CR+ and the CR-
fractions of TDL and it was established that the response transferred by
CR- cells did not arise from either a raidoresistant B cell in the host or
from CR+ cells contaminating the CR- population. Thus, the C3 receptor is
not obligatory for B-cell-T- cell cooperation in the 7S response. The
precursors of 19S antibody- forming cells were found only in the CR+
subpopulation. The CR-Ig+ subpopulation was shown to contain all the B
blasts in rat TDL and a very small number (approximately 1% of all TDL) of
small lymphocytes. This latter population contained the CR- 7S precursors
and contributed approximately 20% of the total adoptive secondary 7S
response transferred by CR+ and CR- subpopulations combined. This
observation suggests that the percentage of rat TDL committed to carry 7S
memory is small, a conclusion which is confirmed and extended in the
following paper.
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