Pathogenesis of of cytomegalovirus infection. I. Activation of virus from bone marrow-derived lymphocytes by in vitro allogenic reaction

doi:10.1084/jem.141.3.561

This Article

	Full Text (PDF)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Olding, L. B.
	Articles by Oldstone, M. B.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Olding, L. B.
	Articles by Oldstone, M. B.


Social Bookmarking

	What's this?

ARTICLES

Pathogenesis of of cytomegalovirus infection. I. Activation of virus from bone marrow-derived lymphocytes by in vitro allogenic reaction

LB Olding, FC Jensen and MB Oldstone

After infection in utero or at birth with a cell culture adapted strain of mouse cytomegalovirus (MCMV), several mouse strains developed a latent virus infection in the presence of specific antiviral antibodies. Up to 5 mo after infection, MCMV could be activated and recovered from spleen lymphocytes of the infected animals that were co- cultivated with histoincompatible (H-2 foreign) mouse embryo cells from uninfected animals. In contrast, co-cultivation of lymphoid cells from infected mice with mouse embryo cells from syngeneic, histocompatible (H-2 similar) donors did not activate MCMV. Similarly, MCMV was not recovered from sonicated lymphoid cells. Virus was activated by treating viable lymphoid cells with lipopolysaccharide, a B-cell mitogen, but was not activated by a variety of other mitogens such as phytohemagglutinin, concanavalin A, or pokeweed mitogen. Subsequent purification of lymphoid cells from the infected mice by a variety of techniques indicated that MCMV was harbored in the B-lymphocyte population.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Kotton, C. N., Fishman, J. A. (2005). Viral Infection in the Renal Transplant Recipient. J. Am. Soc. Nephrol. 16: 1758-1774 [Abstract] [Full Text]
Mueller, N. J., Barth, R. N., Yamamoto, S., Kitamura, H., Patience, C., Yamada, K., Cooper, D. K. C., Sachs, D. H., Kaur, A., Fishman, J. A. (2002). Activation of Cytomegalovirus in Pig-to-Primate Organ Xenotransplantation. J. Virol. 76: 4734-4740 [Abstract] [Full Text]
Collier, A. C., Kalish, L. A., Busch, M. P., Gernsheimer, T., Assmann, S. F., Lane, T. A., Asmuth, D. M., Lederman, M. M., Murphy, E. L., Kumar, P., Kelley, M., Flanigan, T. P., McMahon, D. K., Sacks, H. S., Kennedy, M. S., Holland, P. V., for the Viral Activation Transfusion Study Group, (2001). Leukocyte-Reduced Red Blood Cell Transfusions in Patients With Anemia and Human Immunodeficiency Virus Infection: The Viral Activation Transfusion Study: A Randomized Controlled Trial. JAMA 285: 1592-1601 [Abstract] [Full Text]
Akiyoshi, D. E., Denaro, M., Zhu, H., Greenstein, J. L., Banerjee, P., Fishman, J. A. (1998). Identification of a Full-Length cDNA for an Endogenous Retrovirus of Miniature Swine. J. Virol. 72: 4503-4507 [Abstract] [Full Text]
Wu, B., Dowling, J., Armstrong, J., Ho, M (1975). Enhancement of mouse cytomegalovirus infection during host-versus-graft reaction. Science 190: 56-58 [Abstract]