The Journal of Experimental Medicine
Accuri Cytometers
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The Journal of Experimental Medicine, Vol 140, 1085-1096, Copyright © 1974 by The Rockefeller University Press

ARTICLE

EVIDENCE FOR A CYTOLYTIC FACTOR RELEASED BY MACROPHAGES

Haakon Melsom 1, Gilla Kearny 1, Stanislav Gruca 1, and Rolf Seljelid 1

1 From Norsk Hydro's Institute for Cancer Research, Montebello, Oslo 3, Norway and the Institute for Medical Biology, University of Tromsø, Tromsø, Norway

Mouse peritoneal macrophages cultivated in vitro acquire a strong extracellular cytotoxic activity towards isotope labeled syngeneic erythrocytes as demonstrated by isotope release to the medium. This lytic process is mediated by an extremely labile macrophage cytolytic factor (MCF) which is not detected under ordinary tissue culture conditions with serum present in the medium. By the use of serum-free medium containing low doses of 2-mercaptoethanol MCF is stabilized and found to be an easily dialysable, low molecular substance which resists heating at 60°C for 30 min.

Submitted on July 10, 1974


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