THE INTERACTION OF IgE WITH RAT BASOPHILIC LEUKEMIA CELLS: I. EVIDENCE FOR SPECIFIC BINDING OF IgE

doi:10.1084/jem.139.3.600

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ARTICLE

THE INTERACTION OF IgE WITH RAT BASOPHILIC LEUKEMIA CELLS : I. EVIDENCE FOR SPECIFIC BINDING OF IgE

Anthony Kulczycki Jr. ¹, Chaviva Isersky ¹, and Henry Metzger ¹

¹ From the Section on Chemical Immunology, Arthritis and Rheumatism Branch, National Institute of Arthritis, Metabolism and Digestive Diseases, National Institutes of Health, Bethesda, Maryland 20014

A rat basophilic leukemia cell line originally described byEccleston et al. (3) has been successfully transplanted intoeight Wistar strains and adapted to suspension cell culturewithout noticeable morphological changes.

The cells depletethe reaginic activity of mouse and rat immune sera to an extentroughly equivalent to that reported for normal rat mast cells.Studies utilizing radioiodinated antilight-chain antibodiesand radioiodinated partially purified rat IgE indicate thatthe cells bind IgE to their surface membrane with high specificity.Heating or mildly reducing the rat IgE destroyed its bindingactivity. The binding is unaffected by the presence or absenceof Ca⁺⁺ and Mg⁺⁺, and is markedly inhibited by reaginic butnot by normal rat or mouse serums. The affinity of these cellsfor human IgE, if present at all, is substantially lower thanfor rat IgE.

Submitted on November 11, 1973

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