The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 139, 439-444, Copyright © 1974 by The Rockefeller University Press

BRIEF DEFINITIVE REPORTS

SPECIFIC HETEROLOGOUS ENHANCEMENT OF IMMUNE RESPONSES : VI. PARTIAL PURIFICATION OF A NONSPECIFIC ENHANCING FACTOR FROM SUPERNATES OF ALLOGENEICALLY STIMULATED HUMAN LYMPHOCYTE CELL LINES



Arnold S. Rubin 1, A. Bruce MacDonald 1, and Albert H. Coons 1

1 From the Department of Pathology, Harvard Medical School, and the Department of Microbiology, Harvard University School of Public Health, Boston, Massachusetts 02115

The mixing of two histoincompatible human lymphocyte cell lines generated the release of a soluble factor which was capable of nonspecifically enhancing the in vitro immune response of normal mouse spleen cells against sheep erythrocytes. When active supernates were subjected to exclusion chromatography on Sephadex G-150 and BioGel P-150, the active principle eluted with molecules of approximately 35,000 mol wt. Column aliquots from similarly treated supernates from independent cultures of each lymphoid cell line were inactive. The human enhancing factor was concentrated and purified by ammonium sulfate fractionation, followed by Sephadex gel filtration and polyacrylamide gel electrophoresis.

Submitted on November 18, 1973


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