The Journal of Experimental Medicine
Accuri Cytometers
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The Journal of Experimental Medicine, Vol 138, 1282-1287, Copyright © 1973 by The Rockefeller University Press

BRIEF DEFINITIVE REPORTS

AN ELECTROPHORETIC DIFFERENCE BETWEEN SURFACE AND SECRETED IGM OF MURINE SPLENOCYTES

Ulrich Melcher 1 and Jonathan W. Uhr 1

1 From the Department of Microbiology, University of Texas Southwestern Medical School, Dallas, Texas 75235

µ-chains on the surface of murine splenocytes are more heterogeneous on SDS-polyacrylamide gel electrophoresis than both secreted and intracellular µ-chains labeled with [3H]tyrosine. No difference in heterogeneity among cell surface, secreted, or intracellular L chains was detected. The possible role of carbohydrate in µ-chain heterogeneity is discussed.

Submitted on August 28, 1973


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