The Journal of Experimental Medicine, Vol 138, 1270-1275,
Copyright © 1973 by The Rockefeller University Press
A NEW SURFACE MARKER ON T LYMPHOCYTES OF HUMAN PERIPHERAL BLOOD
Sten Hammarström 1,
Ulla Hellström 1,
Peter Perlmann 1, and
Marie-Louis Dillner 1
1 From the Department of Immunology, The Wenner-Gren Institute, University of Stockholm, S-113 45 Stockholm, Sweden
Neuraminidase treatment of human peripheral blood lymphocytes uncovers cell surface receptors that bind purified A hemagglutinin from the snail Helix pomatia. No hemagglutinin was bound to untreated lymphocytes. Binding studies with 125I-labeled hemagglutinin suggested that the number of receptors on neuraminidase-treated lymphocytes was approximately 1·106/cell. The apparent association constant for hemagglutinin binding to lymphocytes, as calculated from Scatchard's plots, was 5–7·108 liters/mol.
Immunofluorescent staining with FITC-conjugated hemagglutinin gave positive reactions with approximately 60% of the lymphocytes from normal donors. Positive staining was inversely related to the number of lymphocytes with Fc or complement receptors or with surface immunoglobulin, thus suggesting that
See PDF for Structure
the lymphocytes with receptors for Helix pomatia A hemagglutinin are T cells. Cell fractionation on columns charged with hemagglutinin indicate that these receptors may be used for separating subpopulations of human peripheral lymphocytes.
Submitted on July 5, 1973
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
