REQUIREMENT FOR -L-FUCOSE ON THE MACROPHAGE MEMBRANE RECEPTOR FOR MIF

¹ From the Departments of Medicine and Biological Chemistry, Harvard Medical School and Robert B. Brigham Hospital, Boston, Massachusetts 02120

-L-fucose abolishes the activity of guinea pig migration inhibitory factor (MIF) on the macrophages. Other sugars such as -D-glucose, ß-D-galactose, -L-rhamnose, methyl--D-mannoside, and N-acetyl-ß-D-glucosamine had no effect. Theabolition of MIF activity by -L-fucose was reversible. When macrophages were incubated with -L-fucosidase, a glycosidase which splits terminal -L-fucose from oligosaccharides, the macrophages no longer responded to MIF. On the other hand, MIF incubated with -L-fucosidase was still active. These experiments strongly suggest that -L-fucose comprises an essential part of a macrophage membrane receptor for MIF.

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