The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 138, 394-409, Copyright © 1973 by The Rockefeller University Press

ARTICLE

SURFACE IgE ON HUMAN BASOPHILS DURING HISTAMINE RELEASE

Karl E. Becker 1, T. Ishizaka 1, H. Metzger 1, K. Ishizaka 1, and Philip M. Grimley 1

1 From the Arthritis and Rheumatism Branch, National Institute of Arthritis, Metabolism, and Digestive Diseases, National Institutes of Health, Bethesda, Maryland 20014; the Departments of Microbiology and Medicine, Johns Hopkins University School of Medicine, Good Samaritan Hospital, Baltimore, Maryland 21212; and the Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014

The distribution of surface IgE on human basophils was studied using fluorescence microscopy and immunoferritin electronmicroscopy. Redistribution of the IgE was dose, time and temperature dependent and required divalent anti-IgE. Cells which can release histamine in vitro were indistinguishable in these respects from cells which cannot. The redistribution was unaffected by the presence or absence of Ca++.

No correlation between the conditions required for optimal histamine release and for redistribution was observed. At low doses, optimal histamine release occurred in the absence of, or before, redistribution. At higher doses redistribution occurred in the absence of histamine release. Antigen-induced histamine release was unaccompanied by gross redistribution of the surface IgE.

Since both histamine release and redistribution require bridging of IgE on basophils it is concluded that only certain kinds of cross-linking of the IgE effectively stimulates these cells.

 Submitted on May 1, 1973


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