The Journal of Experimental Medicine, Vol 138, 259-277,
Copyright © 1973 by The Rockefeller University Press
COMPARATIVE IMMUNOGENICITY AND ENHANCEABILITY OF INDIVIDUAL H-2K AND H-2D SPECIFICITIES OF THE MURINE HISTOCOMPATIBILITY-2 COMPLEX
Ian F. C. McKenzie 1 and
George D. Snell 1
1 From the Transplantation Unit and the General Surgical Services, Massachusetts General Hospital, and the Department of Surgery, Harvard Medical School, Boston, Massachusetts, 02114, and the Jackson Laboratory, Bar Harbor, Maine, 04609
The immunogenicity of single, private H-2 specificities has been tested. In most cases, the specificity was known to be confined either to H-2K or to H-2D. This was accomplished by the appropriate choice, as donor and recipient, or parent of the F1 hybrid recipient, of congenic strains differing at H-2 only, and of recombinant haplotypes in donor and/or recipient.
By nearly all tests, the H-2K antigen appeared to be a stronger immunogen than the H-2D antigen. Skin grafts with an H-2K difference showed median survival times (MST) of 9.3–14.5 days; for H-2D the values were 13.8–18.6. The difference was also present, though narrowed, for second-set grafts. H-2K grafts regularly engendered a demonstrable cytotoxic antibody response; with H-2D differences the response was absent or very weak. K end cytotoxic titers after multiple immunizations with lymphoid tissues ranged from 1/32 to 1/2,048, D end titers from 0 to 1/128. Hemagglutination titers showed no clear difference.
The results of passive enhancement of skin grafts with H-2 alloantibody produced in donor recipient combinations, identical to those used for the skin grafts showed a different pattern. H-2K, despite its greater immunogenic strength was more easily enhanced than H-2D. Prolongation of MST's for H-2K was 2.4–6.7 days, for H-2D grafts, 0.2–1.5 days.
Submitted on March 21, 1973
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