J. N. Ihle ¹, M. Yurconic Jr. ¹, and M. G. Hanna Jr. ¹

¹ From the Carcinogenesis Program, Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830

The radioimmune precipitation assay using ³H-labeled AKR leukemia virus was applied to the detection and quantitation of natural serum antibodies directed against endogenous murine leukemia virus (MuLV) envelope antigens B6C3F₁ and BALB/c mice, which have low natural incidences of leukemia and lymphoma, and AKR mice, which have a high incidence, were used in this study. Sera from mice of various age groups were assayed. A marked difference in age-associated levels of the autogenous immune response to endogenous murine leukemia virus was detected, and the quantitative differences among these strains were inversely related to the incidence of lymphoma. The radioimmune precipitation test as applied was 500 times more sensitive than virus neutralization. That the reactions we have observed are specific is suggested by several lines of evidence, including the nonreactivity of normal hamster and absorbed rat serum, the positive reaction of absorbed rat anti-AKR serum, the inhibition of precipitation of labeled virus by purified unlabeled virus, and isopycnic gradient analysis of the reactive products.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Ihle, J., Joseph, D., Domotor, J. Jr (1979). Genetic linkage of C3H/HeJ and BALB/c endogenous ecotropic C-type viruses to phosphoglucomutase-1 on chromosome 5. Science 204: 71-73 [Abstract]  
• Aoki, T, Liu, M, Walling, M., Bushar, G., Brandchaft, P., Kawakami, T. (1976). Specificity of naturally occurring antibody in normal gibbon serum. Science 191: 1180-1183 [Abstract]  
• Nowinski, R. C., Kaehler, S. L. (1974). Antibody to Leukemia Virus: Widespread Occurrence in Inbred Mice. Science 185: 869-871 [Abstract]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS