The Journal of Experimental Medicine
StemCell Technologies
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The Journal of Experimental Medicine, Vol 137, 1311-1324, Copyright © 1973 by The Rockefeller University Press

ARTICLE

ACTIVE SUPPRESSION OF IMMUNOGLOBULIN ALLOTYPE SYNTHESIS : III. IDENTITICATION OF T CELLS AS RESPONSIBLE FOR SUPPRESSION BY CELLS FROM SPLEEN, THYMUS, LYMPH NODE, AND BONE MARROW



Leonore A. Herzenberg 1, Eva L. Chan 1, Myrnice M. Ravitch 1, Roy J. Riblet 1, and Leonard A. Herzenberg 1

1 From the Department of Genetics, Stanford University School of Medicine, Stanford, California 94305

Thymus-derived cells (T cells) that actively suppress production of IgG2a immunoglobulins carrying the Ig-1b allotype have been found in adult (SJL x BALB/c)F1 mice exposed to anti-Ig-1b early in life. The suppression is specific for Ig-1b. The allelic product, Ig-1a, is unaffected.

Spleen, lymph node, bone marrow, or thymus cells from suppressed mice suppress production of Ig-1b by syngeneic spleen cells from normal F1 mice. When a mixture of suppressed and normal cells is transferred into lethally irradiated BALB/c mice, there is a short burst of Ig-1b production after which Ig-1b levels in the recipient fall rapidly below detectability. Pretreatment of the cells from the suppressed mice with antiserum specific for T cells (anti-Thy-1b) plus complement before mixture destroys the suppressing activity.

Similar results with suppressor cells were obtained in vitro using Mishell-Dutton cultures. Mixture of spleen cells from suppressed animals with sheep erythrocyte (SRBC)-primed syngeneic normal spleen before culture suppresses Ig-1b plaque-forming cell (PFC) formation while leaving Ig-1a PFC unaffected. Treatment of the suppressed spleen with anti-Thy-1b before transfer removes the suppressing activity.

 Submitted on February 5, 1973


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